49 JPA | Volume 4 | Issue 3 | September, 2015 Research & Reviews: Journal of Pharmaceutical Analysis INTRODUCTION India has a rich heritage of traditional medicine. Materia medica of India provides a lot of information on the folklore practices and traditional aspects of therapeutically important natural products India has a rich heritage of traditional medicine which constitutes different components like Ayurveda, Siddha and Unani. The development of these traditional systems of medicines with the perspective of safety, effcacy, and quality will not only help to preserve the traditional heritage but also to rationalize the use of natural products in healthcare [1] . Unconventional medicine has become more popular in recent years [2] . Natural products have been our single most successful source of medicine. The use of plant compounds for pharmaceutical purposes has gradually increased worldwide. The research and development thrust in the pharmaceutical sector is focused on the development of new innovative/indigenous plant-based drugs through the investigation of leads from the traditional system of medicine [3] . Each plant is like a chemical factory capable of synthesizing a limited number of highly complex and unusual chemical substances derived from plants that are considered as important drugs currently in use, while several other drugs are simple synthetic modifcations of the natural products [4] . Mangifera indica L. (Anacardiaceae) is one of the most important and abundant tropical fruit in the world and India contributes to a major part of the world’s mango production. In Indian delicacy, mango is considered as the king of all fruits. Mangifera indica L. (Anacardiaceae) is one of the most important tropical fruits in the world, as some 69 varieties of the species are known [5] and India contributes Isolation of Mangiferin from Flowering Buds of Mangifera indica L and its Evaluation of in vitro Antibacterial Activity Taniya Biswas, Argha Sen, Rini Roy, Sushomasri Maji, Himangshu Sekhar Maji* Department of Pharmaceutical Analysis, Bengal School of Technology Sugandha, Delhi Road, Hooghy-712102, West Bengal, India Research Article ABSTRACT Mango (Mangifera indica L) which belongs to the family of Anacardiaceae, is a rich source of biologically active compound mangiferin, which is a natural xanthone C-glucoside. Mangiferin, (1,3,6,7-tetrahydroxy xanthone-C2-b-D- glucoside), has been isolated from various parts of Mangifera indica. Mangiferin is a pharmacologically active phytochemical present in large amount in bark, fruits, roots and leaves of Mangifera indica. Mangiferin has been traditionally used in some parts of world as anti-infammatory, antibacterial, analgesic, antipyretic, antioxidant, antitumor, antiviral, immunomodulatory, anthelminthic and in obesity treatment. The present work is aimed to isolate mangiferin by column chromatography from the ethanolic extract of fowering buds of Mangifera indica L and assess its antibacterial activity. The conclusive structure of the isolated compound was established using TLC, HPLC, UV/VIS, FTIR and NMR spectral analysis. In vitro antibacterial activity of the isolated mangiferin was studied on Gram positive and Gram negative bacteria by agar disc-diffusion techniques. The solutions of the isolated mangiferin showed activity with regard to various strains of two bacterial species: Staphylococcus aureus (Gram positive) and Salmonella typhi (Gram negative). The present study confrms the antibacterial effect of the isolated mangiferin which could be further processed for its development as an antibacterial agent. Received date: 23/05/2015 Accepted date: 25/08/2015 Published date: 01/09/2015 *For Correspondence Himangshu Sekhar Maji, Department of Pharmaceutical Analysis, Bengal School of Technology Sugandha, Delhi Road, Hooghly-712102, West Bengal, India E-mail: hsmaji77@gmail.com Keywords: Glucoside; Ethanolic; Antibacterial; Agar disc-diffusion; Gram positive