International Journal for Innovation Education and Research ISSN 2411-2933 01 May 2021 International Journal for Innovation Education and Research© 2021 pg. 478 Safety profile, antimicrobial and antibiofilm activities of a nanostructured lipid carrier containing oil and butter from Astrocaryum vulgare: in vitro studies Aline Rossato a , Larissa da Silva Silveira a , Pâmella Scharamm Oliveira a , Thobias Toniolo de Souza b , Ana Paula Becker b , Roger Wagner c , Bruna Klein c , Walter Paixão de Souza Filho a , Roberto Christ Vianna dos Santos d , Diego de Souza a , Matheus Dellaméa Baldissera e , Michele Rorato Sagrillo a a Graduate Program in Nanosciences, Universidade Franciscana, Santa Maria, RS, Brazil. b Biomedicine Curse, Universidade Franciscana, Santa Maria, RS, Brazil. c Department of Technology and Food Science, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul state, Brazil. d Graduate Program of Pharmaceutical Sciences, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. e Graduate Program of Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Correspondence: Dr. Michele Rorato Sagrillo, Program in Nanosciences, Universidade Franciscana, Santa Maria, RS, Brazil. E-mail: sagrillorm18@gmail.com. Abstract Ethnopharmacological relevance: Tucumã (Astrocaryum vulgare)is a fruit native to the Amazon region. Extracts from the peel and pulp are thought of as promising treatments for bacterial infections. The primary constituents of Tucumã oil and butter possess unsaturated carbon chains that are susceptible to oxidation by light or heat. The oils have high volatility and low aqueous solubility that limits their use without a vehicle. Nanotechnology refers to techniques to solve these problems. Nanostructured lipid carriers (NLC), for example, protect fixed oils degradation by heat or light, as well as from oxidation and evaporation, ensuring greater stability and function, thereby prolonging the useful life of the final product. Study objectives: The objective of this study was to evaluate the hemolytic, cytotoxic, antimicrobial and antibiofilm properties of an NLC containing Tucumã butter and oil soasto improve the solubility and photosensitivity of the compounds, generating better pharmacological efficacy. Materials and methods: The NLC was assessed for stability for 60 days. The cytotoxicity of nanoparticles in peripheral blood mononucleated cells was determined in culture using assays for cell viability, DNA damage, oxidative metabolism and damage to human erythrocytes. Antimicrobial activity was determined using the broth microdilution technique and antibiofilm activity according to standardized protocols. Results: The Tucumã NLC remained stable throughout the evaluated period, with pH between 5.22–5.35, monodisperse distribution (PDI<0.3) and average particle size of 170.7 ± 3nm. Cytotoxicity studies revealed that the NLC is safe and modulates inflammatory processes, demonstrated by increased cell viability and nitric oxide levels. There was low hemolytic activity of the NLC against human erythrocytes atmost concentrations