ARC Journal of Dental Science Volume 1, Issue 3, 2016, PP 9-14 ISSN No. (Online): 2456-0030 http://dx.doi.org/10.20431/2456-0030.0103003 www.arcjournals.org ©ARC Page | 9 Study on the Effect of Ethanol Extract of Ipomoea Aquatica (Kalmi Shak) Leaves on Gentamicin Induced Nephrotoxic Rats Dr. Rayhana Sharmin. MBBS, M Phil. (Pharmacology) 1 , Dr. A.B.M.Iftekhar Hossain, MBBS. (Pathology) 2 , Dr. Sharmin Rahman. MBBS, M Phil. (Pharmacology) 3 , Dr. Azmary Momtaz. MBBS, M Phil. (Pharmacology) 4 , Dr. Khaleda Sharmin. MBBS, M Phil. (Pharmacology) 5 , Dr. Abu Syed Md. Mosaddek, MBBS, M Phil. (Pharmacology) 6 1 Lecturer, Department of Pharmacology and Therapeutics, Uttara Adhunik Medical College, Dhaka, Bangladesh 2 Department of Pathology, Mymensingh Medical College 3 Assistant Professor, Department of Pharmacology and Therapeutics, Ibrahim Medical College, Dhaka, Bangladesh 4 Assistant Professor, Department of Pharmacology and Therapeutics, Delta Medical College, Dhaka, Bangladesh 5 Assistant Professor, Department of Pharmacology and Therapeutics, University Dental College, Dhaka, Bangladesh 6 Professor and head, Department of Pharmacology and Therapeutics, Uttara Adhunik Medical College, Dhaka, Bangladesh rayhanasharmin14@gmail.com, ifte_33@yahoo.com, sharmin.rahman80.srs@gmail.com, lizasomc@yahoo.com, dr.ksharmin@yahoo.com, Drmosaddek25@gmail.com Abstract: Background: Drug-induced kidney disease constitutes an important cause of acute renal failure. Recent studies indicate that free radicals are important mediators of kidney injury induced by gentamicin (GM), an aminoglycoside antibiotic widely used in treating severe gram-negative infection. Ipomoea aquatica (IA) was reported to have antioxidant and free redical scavenging activities. Objective: The present study was performed to find out the nephroprotective effect of Water spinach IA ethanol extract leaves On Gentamicin-induced nephrotoxic rats. Materials and methods: The experiment was carried out in the department of Pharmacology of Dhaka Medical College, Dhaka, from January 2013 to December 2013. For the purpose of the study 32 wistar albino rats either both sex were taken and divided into four groups (Gr-I, Gr-II, Gr-III and Gr-IV) consisting 8 rats each. Group I received laboratory diet and distilled water for 14 days. Group II received IA ethanol extract at the rate of 500 mg/kg body weight orally by gastric intubation for 14 days. Group III received normal diet for 14 days and injection gentamicin at a dose of 80 mg/kg/day intraperitoneal from day seven to day fourteen. Group IV received ethanol extract of Ipomoea aquatica 500 mg/kg/day orally from day one to day six,then received concomitant treatment with injection gentamicin at a dose of 80 mg/kg/day intraperitoneal from day seven to day fourteen. Results: The serum creatinine and serum urea levels were significantly (p < 0.01) increased in rats treated with GM (Gr-III) as compared to control group. Histopathology of kidney of this group also showed massive interstitial nephritis. Again Pre & concomitant administration of IA ethanol extract on GM induced nephrotoxic rats (Gr-IV) significantly (p < 0.01) decreased the elevated serum creatinine and urea levels when compared to those of GM treated group. The comparative histopathological study of kidney exhibited almost normal architecture as compared to control group. Conclusion: It may be concluded that, Ipomoea aquatica may protect and cure kidney tissues against nephrotoxic effect of gentamicin, though accurate mechanism and safety profile is not confirmed by this study. Keywords: Gentamicin, Ipomoea aquatic, Serum creatinine, serum urea.