Pharmacologyonline 1: 591-598 (2010) Sivakumar et al. 591 BIO ACTIVITY OF TINOSPORA CORDIFOLIA CRUDE METHANOLIC EXTRACT IN EXPIREMENTAL DIABETES V. SIVAKUMAR 1 *, M.S.DHANA RAJAN 2 , A.MOHAMED SADIQ 3 , M.JAYANTHI 3 1* Department of Pharmacy, Sri Lakshmi Narasimha College of Pharmacy, Palluru-517 132.Andhra Pradesh. India. 2 P.G and Research Department of Biochemistry, Jaya College of Arts and Science, Thiruninravur - 602 024. Tamilnadu. India. 3 P.G and Research Department of Biochemistry, Adhiparasakthi College of Arts and Science, Kalavai, Vellore District - 632 506. Tamilnadu. India. Corresponding author: V. Sivakumar, Department of Pharmacy, Sri Lakshmi Narasimha College of Pharmacy, Palluru - 517 132. Andhra Pradesh. India. Mobile no: + 91 9944929101 E-mail address: sivakumarv2k5@sify.com siva_bio2009@rediffmail.com Summary The traditional system of the medicine remains the major source of the health care. It is safe alternative, lesser cost and better tolerance and its complications. The major defense against free radicals found in medicinal plants is in the form of natural antioxidants. The present investigation is an attempt to asses the bioactivity of daily oral administration of methanolic extract of Tinospora cordifolia stem (T.C.S) (500mg/kg body weight) for 6weeks in normal and alloxan induced diabetic rats. A significant decrease in blood glucose, glycosylated haemoglobin, cholesterol (p<0.05), and increase in body weight, protein (p<0.01) were observed in diabetic rats on treatment with T.C.S. methanolic extract when compared to normal. The activity of the hepatic enzyme hexokinase was significantly increased where as glucose 6-phosphatase and fructose 1, 6- bisphosphatase were significantly decreased (p<0.01) by the oral administration of T.C.S. methanolic extract in diabetic rats when compared to normal. The six weeks treatment of T.C.S. methanolic extract was proved natural antioxidant present in the plant, because the activity of erythrocytes lipid peroxide and catalase (CAT) was significantly (p<0.01) increased whereas superoxide dismutase (SOD) and reduced glutathione (GHS- Px) were significantly (p<0.01) decreased when compared to normal rats. Key words: Tinospora cordifolia, Blood glucose, Cholesterol, Antioxidants, Alloxan diabetes, Methanolic extract.