Indian Journal of Pharmaceutical Education and Research | Vol 54 | Issue 2 (Suppl) | Apr-Jun, 2020 S337 Original Artcle www.ijper.org Ultraviolet-visible Spectrophotometric Method for Estimation of Gliclazide in Presence of Excipients Interacting in UV-visible Region Neelkant Prasad 1, *, Aditya Sharma 2 1 S G T College of Pharmacy, S G T University, Gurugram-Badli Road, Budhera, Gurugram, Haryana, INDIA. 2 School of Pharmaceutical Sciences, IFTM University, Delhi Road, Lodhipur Rajput, Moradabad, Uttar Pradesh, INDIA. ABSTRACT A simple and sensitive ultraviolet spectrophotometric method for quantitative estimation of a model API gliclazide in presence of excipients is described to avoid false estimation due to presence of soluble or insoluble impurity. UV detection was performed at 226 nm, 221 nm and 231 nm and the calibration curve was prepared between the resultant of absorbance at these three wavelengths according to the equation [226 nm - (221 nm + 231 nm)/2] and the concentration of gliclazide. The calibration curve was found to be linear over concentration range tested (04-28 µg/ml) having limit of detection of 0.45 μg/ml and limit of quantifcation 1.36 μg/ml. Percent relative standard deviations, representing precision, for pure as well as impure solutions were found to be within acceptable limits i.e. always less than 1.99 and 2.00 respectively for pure and impure solution. Mean percent recovery of 99.21%-102.04% and 102.06%-103.74% for pure and impure solution respectively indicates that the developed method is accurate. Conclusively, the developed method can be effectively applied for the estimation of gliclazide in pure as well as impure solutions and it was seen that the analyte in both types of solutions can be detected from same calibration curve accurately and precisely. Key words: Pure, Impure, Excipients, UV spectrophotometric method, Gliclazide. DOI: 10.5530/ijper.54.2s.91 Correspondence: Dr. Neelkant Prasad Associate Professor, College of Pharmacy, S G T University, Gurugram-Badli Road, Budhera, Gurugram-122505, Haryana, INDIA. Phone: +91 9027169402 E-mail: prasadneelkant@ gmail.com Submission Date: 28-06-2019; Revision Date: 05-11-2019; Accepted Date: 30-04-2020 INTRODUCTION The samples of dissolution of various dosage forms like tablets, capsules, solid dispersions, gels etc. and samples from solubility studies contain dissolved or undissolved substances that might interact with quantitative estimation of substance of interest until and unless separated. Separation of undissolved impurity can be achieved by fltration 1 and/ or centrifugation 2 while dissolved interacting substance can be separated by chromatographic methods like HPLC. 3 All these separation methods require special technique/s and instrument/s involved with them. These cause multiple increases in the costs as well as the time duration of evaluation. Further, fltration may cause adsorption of analyte drug substance of interest onto the flter medium. 4,5 HPLC requires use of various organic solvents and their combina- tion to achieve complete separation of the interacting substances. Moreover, all these techniques result in consumption of power and energy. Dissolved impurities are sepa- rated by chromatographic columns and the process is tedious, costly and time consum- ing. Derivative spectrophotometry, bichromatic methods and difference spectrophotometry can also be applied for elimination of background light absorption but all these methods are more or less complex. 6 Also, derivative spectrophotometry results in more complex spectrum. 7 Different analytical methods including UV spectrophotometry 4 , gas chromatography 5 , HPLC, 6,7 Evaporative Light Scattering Detection, 8 Charged Aerosol Detection 8