Food Science and Applied Biotechnology, 2022, 5(2), 211-218 Food Science and Applied Biotechnology Journal home page: www.ijfsab.com https://doi.org/10.30721/fsab2022.v5.i2 ✉ Corresponding author: Mariya Brazkova, Technological Faculty, University of Food Technologies, Plovdiv, Bulgaria, tel.: +359 32 603 828; E-mail: mbrazkova@uft-plovdiv.bg Article history: Received 14 June 2022 Reviewed 20 July 2022 Accepted 27 September 2022 Available on-line 04 October 2022 https://doi.org/10.30721/fsab2022.v5.i2.203 © 2022 The Authors. UFT Academic publishing house, Plovdiv Angelova et al., 2022 Effect of the lignocellulose substrate type on mycelium growth… Page 211 Research Article Effect of the lignocellulose substrate type on mycelium growth and biocomposite formation by Ganoderma lucidum GA3P Galena Angelova 1 , Mariya Brazkova 1✉ , Bogdan Goranov 2 1 Department of Biotechnology, Technological Faculty, University of Food Technologies, Plovdiv, Bulgaria 2 Department of Microbiology, Technological Faculty, University of Food Technologies, Plovdiv, Bulgaria Abstract The lignocellulose agricultural wastes, one of the major environmental pollutants, represent an extremely rich resource with high nutritional value, which can be used in the production of value-added products. In the current study the effect of different lignocellulose substrates on the growth rate of Ganoderma lucidum GA3P and the formation of mycelium-based bio-composites was determined. The macromorphology and specific mycelial growth rate of the colonies on different media containing various lignocellulosic substrate were studied. The obtained composites were characterized regarding their density of the mycelial growth, apparent density and size. G. lucidum GA3P demonstrated high μmax values ranging from 0.267 d -1 to 0.558 d -1 and low K values indicating that all used media were suitable for cultivation, but when wheat bran was used, the formed mycelium-based bio-composites possessed the best characteristics with highest apparent density recorded (0.39 ± 0.005). Keywords lignocellulose, Ganoderma lucidum, mycelium-based bio-composites Abbreviations BP – beep presses; HERF – hexane extracted rose flower; MCM – Mushroom complete medium; MR – malt roots; PS – pine sawdust; SDLS – steam distillated lavender straw; WB – wheat bran; WS – wheat straw