1114 Medico-legal Update, April-June 2021, Vol. 21, No. 2 The Efectiveness of Crocodile Oil Extract Ointment on the Treatment of Burns in Mice (Mus musculus) Sarce Makaba 1 , Rosmin M. Tingginehe 1 , Yacob Ruru 2 1 Faculty of Public Health, Cenderawasih University, Jayapura, Papua, Indonesia, 2 Department of Statistics, Faculty of Mathematics and Natural Sciences, Cenderawasih University, Jayapura, Papua, Indonesia Abstract The purpose of this study was to determine the efectiveness of the crocodile oil extract ointment in the treatment of burns in mice (Mus musculus). The research found that the ointment of crocodile oil extract has an efect in the healing of burns in mice (Mus musculus), with the best concentration of 30% of the crocodile oil extract ointment dose. Moreover, the ointment met the standard on the basis of organoleptic and homogeneity tests. The ointment of crocodile oil extract with concentrations of 30% and 50% has the healing activity degree II-A burns in mice (Mus musculus). The best percentage of reductions in average burns in mice was the ointment with a concentration of 30%. Further study needs to be done in the healing of wound in human skin. Keywords: Burn, crocodile oil extract, efectiveness, ointment. Corresponding Author: Sarce Makaba Faculty of Public Health, Cenderawasih University, Jayapura, Papua, Indonesia e-mail: sarcemakaba20@gmail.com Introduction Burns is one of the most common injuries in accidents and continues to be a global public health problem. 1 Burn injuries can lead to complications such as long-term disability, prolonged hospitalization, loss of body limbs and even death. 2 Wound repair is a process that follows skin injury, which is initiated by an infammatory reaction and the cells below the dermis begin to increase the production of collagen. The epithelial tissue is then regenerated. 3 Although many advances have been made in our understanding and in the care of burn injuries, modern medicine still has a challenge to heal burn injuries. 1 Traditional medicine has existed since human civilization and has been growing for thousands of years with the combination of new technology in the search for good drugs. 4,5 This is important for public health, based on the experience and knowledge that has been collected to date. Humans have used medicines for alleviating and treating diseases with natural products such as plants, animals, microorganisms and marine organisms. 6,7 Traditional medicine is one of the nation’s cultural heritages, which is very valuable for the preservation of traditional medicine for public health. The development of the use of natural ingredients as a traditional medicine is now more desirable, as it is relatively easy to obtain. This is generally derived from a wide variety of plants, animals and minerals. Natural materials such as plants, animals and mixtures of these materials may be used as traditional medicines. They contain a variety of compounds that can afect the living cells of the organ. Wound injury healing process is efectively promoted through the use of traditional remedies, mainly based on plant and animal sources. 1 In addition; natural ingredients have minimal side efects. One of the natural ingredients used as a traditional medicine other than plants is derived from animals such as the use of crocodile oil for treatment. Siamese crocodile (Crocodylus siamensis) is one of the freshwater crocodile species that was originally distributed throughout Southeast Asia. 8 Crocodile oil has been shown to be very efective in the treatment of diseases, ranging from skin conditions to cancer, and has been used by traditional practitioners for centuries. 9 In Africa, crocodile oil is used to treat skin rashes and