Mohammadrezabeigi et al. - Bioagrica. Vol. 02(01), 1-9, November 2021 - Article ID: bioagrica-2003312112245 1 Bioagrica 2 (1) 1-9 Article ID : bioagrica-2003312112245 Bioagrica Journal homepage: http://www.htpub.org/Bioagrica/ Diversity of some Iranian isolates of entemopathogenic fungi, Beauveria bassiana by PCR-RFLP rpb 1 & 2 Mohammadrezabeigi, Sahar 1 , Ezatollah Sedaghatfar 2,3  , Arash Nargesy-Dehdasht 3 , Vahid Zarrinnia 4 , Parisa Abdollahi 1 1 Department of Plant Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2 Department of Plant Pathology, Arak Branch, Islamic Azad University, Arak, Iran 3 Research Center for Applied Plant Sciences, Arak Branch, Islamic Azad University, Arak, Iran 4 Department of Plant Pathology, Science and Research Branch, Islamic Azad University, Tehran, Iran Article Information Abstract Article History Received: 14/05/2020 Accepted: 08/10/2021 Available online: 17/11/2021 Entemopathogenic fungi, especially the genus Beauvaria, are naturally the cause of disease and death in various insects. Molecular markers provide a valid method for studying the genetic diversity and population genetic structure of most living organisms, especially entomopathogenic fungi such as B. bassiana. Twelve isolates of Beauveria bassiana were prepared from the soil of 3 different climates of Iran (hot and humid, hot and dry, cold and dry) . Grouping of B. bassiana isolates was performed using PCR-RFLP markers in rpb1 and rpb2 regions. In order to determine the genetic diversity of isolates after extraction of genomic DNA, the amplified DNA was cut with specific primers rpb1 and rpb2 using Hind III restriction enzyme and the pattern of polymorphic bands in agarose gels was 0 And 1 were drawn using MVSP software version 3.22. Trees obtained from rpb1 and rpb2 amplified fragments were plotted in using UPGMA clustering method using Simpson's Coefficient. In clustering obtained from rpb1, except for Ahvaz isolates, all isolates in the hot and humid climate in the south of the country; Andimeshk, Poldakhtar and Khorramabad, Yazd and Kerman from Hot and dry climate were grouped. This study, a pattern was not obtained in accordance with the geographical pattern, nevertheless, special results were obtained. For example, the isolates of Kerman and Yazd in both genes were placed next to each other and Boroujerd, Robat Karim and Poldakhtar were placed in the same procedure. Keywords Beauveria bassiana Genetic diversity rpb1 & rpb2 PCR-RFLP  Corresponding author email address: e-sedaghatfar@iau-arak.ac.ir 1. Introduction Entomopathogenic fungi infect their hosts through the external cuticle and are pathogenic to insects. They have been widely evaluated as control agents for a diverse variety of noxious arthropods of agricultural (including forestry and livestock) and horticultural importance (Glare et al., 2010; Goettel et al., 2010). Fungus Beauveria bassiana are commonly found in both cultivated and non- cultivated soils, although their natural distribution appears to be related to habitat (Meyling et al., 2009). A variety of molecular techniques such as RFLP, T-RFLP, AFLP and strain-specific microsatellite markers have been used as diagnostic tools allowing fungi to be