_____________________________________________________________________________________________________ *Corresponding author: E-mail: ejike.orji@unizik.edu.ng; Asian Journal of Research in Biochemistry 8(4): 1-9, 2021; Article no.AJRB.69582 ISSN: 2582-0516 Extraction and Comparative Characterization of Oils from Edible Seeds of Glycine max and Sesamum indicum Victor Henry Azubuike Enemor 1 , Ejike Celestine Orji 1* , Uchechukwu Chibuzo Ogbodo 1 , Ogechukwu Frances Nworji 1 and Chinaza Lucy Ibeneme 1 1 Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Author VHAE designed and supervised the study. Authors CLI and OFN managed and performed the experimental and statistical aspects of the study. Authors UCO wrote the protocol while ECO wrote the first draft of the manuscript. All authors read and approved the final manuscript. Article Information DOI: 10.9734/AJRB/2021/v8i430185 Editor(s): (1) Dr. Mohamed Fawzy Ramadan Hassanien, Zagazig University, Egypt. Reviewers: (1) Khanin Pathak, Assam Agricultural University, India. (2) Kiran Dahiya, Pt. B. D. Sharma University of Health Sciences, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/69582 Received 10 April 2021 Accepted 14 June 2021 Published 22 June 2021 ABSTRACT Aim: The incidence of heart-related disease conditions due to consumption of cholesterol containing oils and the increasing global demand for oil for domestic and industrial purposes have necessitated the need for scientific evaluation of other neglected indigenous plants for potential quality oil yield. This study thus aimed at extracting and comparing physicochemical and nutritional properties of seed oils from Glycine max and Sesamum indicum with a view to diversifying alternative sources of oil to meet teeming industrial and health needs and for food security. Methodology: Solvent extraction method was employed to extract oil from seed samples which were further subjected to estimation of physicochemical indices such as free fatty acid, saponification value, iodine value, peroxide value, specific gravity, refractive index, density, pH, melting temperature and viscosity according to methods described by using titration method. Results: Findings indicated higher saponification (412.33 mgKOH/mg), acid (2.99 mgKOH/g), free fatty acid (1.49 mgKOH/g), viscosity (0.13 Pas) and melting point (5.66 o C) values for sesame seed oil than for soybean seed oil. However, soybean seed oil showed higher density (0.837 g/ml), Original Research Article