_____________________________________________________________________________________________________ *Corresponding author: E-mail: anulika.onyemelukwe@unn.edu.ng; Journal of Complementary and Alternative Medical Research 19(1): 1-7, 2022; Article no.JOCAMR.90384 ISSN: 2456-6276 Effects of Aqueous and Methanol Extracts of Cocos nucifera (Coconut) Milk on Testosterone Level and Testes of Male Albino Rats Anulika Obianuju Onyemelukwe a* and Chidera Queen Nweze a a Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Author AOO came up with the idea for the study, created the protocol, carried out the investigations, examined the literature critically, proofread the first draft of the manuscript, and wrote the final manuscript. Author CQN was in charge of the data collection and handled the literature searches. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JOCAMR/2022/v19i130365 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/90384 Received 25 June 2022 Accepted 14 August 2022 Published 19 August 2022 ABSTRACT Aims: This study was designed to investigate the effect of aqueous and methanol extracts of Cocos nucifera (coconut) milk on the testosterone levels and testes of male albino wistar rats. Study Design: A 15-day experimental study. Place and Duration of Study: Animal House and Department of Medical Laboratory Science, College of Medicine, University of Nigeria, Enugu Campus, between May 2019-November 2019. Methodology: Twenty five adult male albino rats were divided into five groups (I-V) of five rats each. Rats in group I received only water and standard feed throughout the experiment and served as control. Groups II and III received 163mg/kg and 326mg/kg of methanol extract of coconut milk (MECM) respectively, while group IV and V received 110mg/kg and 220mg/kg of aqueous extract of coconut milk (AECM) for two weeks. Upon completion of extract administration, the blood samples of the rats were obtained by a retro-orbital puncture of the median canthus for estimation of serum testosterone. The animals were sacrificed and the testes excised, weighed and fixed in 10% formal saline for 48hours prior to paraffin embedding for histological processing. Testes sections were Original Research Article