65 Ethanol Extract of Miana Leaf (Coleus atropurpureus Benth) as Analgetic Antiinflamation in Rats (Rattus novergicus) (Ekstrak Etanol Daun Miana (Coleus atropurpureus Benth) sebagai Analgetik Antiinflamasi pada Tikus (Rattus novergicus)) Sukmawati * , Irma Santi, Aulia Wati, Riska Aulya Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Muslim Indonesia, Makassar, Indonesia, 90231 Email: sukmawati.sukmawati@umi.ac.id Article Info: Received: 1 November 2021 in revised form: 12 December 2021 Accepted: 8 March 2022 Available Online: 29 March 2022 ABSTRACT Background: Inflammation is a normal protective response to tissue injury caused by physical trauma, chemicals, or microbiological substances. The manifestation clinic of inflammation is pain. Miana leaves (Coleus atropurpureus Benth.) have chemical contents such as essential oils, phenols, flavonoids, and polyphenols. Flavonoids can protect lipid membranes from damage and inhibit cyclooxygenase, which is the first pathway for the synthesis of pain mediators such as prostaglandins. Objectives: This study aims to determine the effectiveness of ethanol extract of Miana leaves (Coleus atropurpureus Benth) (EEML) as analgesic and anti-inflammatory in rats (Rattus novergicus). Material and Methods: This study used The Pre-Posttest-Only Control Group Design, twenty male rats were divided into 5 groups namely negative control, positive control (diclofenac sodium), treatment with EEML dose of 150, 300, and 600 mg/kg body weight (BW). Anti-inflammatory was assayed by using the rat hind paw edema method where the condition of inflammation in rats is induced by 5% egg white by sub plantar. The measurement of edema in rat feet was using a caliper every 30 minutes for 5 hours. While The measurement of analgesic testing was using the nociception test method which evaluates pain responses such as staggering gait, vocalization, and writhing. The inflammatory parameters data were analyzed with the one-way anova for the width of edema foot and kruskal walis for the thickness of edema foot. While the observing pain response data were analyzed with the Kruskal Walis test. Results: The ethanol extract of Miana leaves has analgesic anti- inflammatory activity especially at a dose of 600 mg/kg bw which are non- significant (p> 0.05) with a positive control group (sodium diclofenac). Conclusions: This study concluded the potency of Miana leaves as analgetic anti-inflammatory drugs. Keywords: Antiinflamation Analgetic Coleus atropurpureus Benth Miana Leaf Rattus novergicus Corresponding Author: Sukmawati Program Studi Sarjana Farmasi Fakultas Farmasi Universitas Muslim Indonesia Makassar 90231 Indonesia email: sukmawati.sukmawati@umi.ac.id Copyright © 2019 JFG-UNTAD This open access article is distributed under a Creative Commons Attribution (CC-BY-NC-SA) 4.0 International license. How to cite (APA 6 th Style): Sukmawati, Santi, I., Wati, A., Aulya, R. (2022). Ethanol Extract of Miana Leaf (Coleus atropurpureus Benth) as Analgetic Antiinflamation in Rats (Rattus novergicus). Jurnal Farmasi Galenika: Galenika Journal of Pharmacy (e-Journal), 8(2), 65- 74. doi:10.22487/j24428744.2022.v8.i1.15678 Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) 2022; 8 (1): 65 – 74 ISSN: 2442-8744 (electronic); 2442-7284 (printed) Journal Homepage: https://bestjournal.untad.ac.id/index.php/Galenika DOI: 10.22487/j24428744.2022.v8.i1.15678