P533 / #111, E-POSTERS TOPIC: 4. CLINICAL VASCULAR DISEASE / 4.01 COAGULATION AND THROMBOSIS. PROTECTIVE EFFECT OF APELIN, AMLODIPINE AND ANAKINRA IN ISCHEMIA-REPERFUSION INJURY IN MYOCARDIUM E.K. Fahmy . Northern Border University (NBU), Physiology, Arar, Saudi Arabia Background and Aims: The aim of this research is to clarify and compare the protective effects of Apelin, Amlodipine (long-acting dihydropyridine- type (DHP) calcium channel blocker) and Anakinra (Kineret-Interleukin-1 Receptor Antagonist) on ischemia- reperfusion injury in myocardium. Methods: A total number of 50 healthy adult male albino rats were used to study and compare the effect of Apelin-13 (injected intraperitoneally at a dose of 1 mg/kg 15 min before reperfusion began), Amlodipine (adminis- tered at a dose of 1 mg /kg body weight daily by oral route for 7 days) and Anakinra (a single intravenous shot of 2mg/kg body weight) on preventing myocardial reperfusion injury in experimental animals. Results: The results of this study showed that in Apelin, Amlodipine and Anakinra treated groups, the mean values of all test parameters were significantly lower than ischemia /reperfusion group(p <0.001). In Ana- kinra treated group, the mean values for all parameters were found to be significantly lower when compared with that Apelin and Amlodipine treated groups. In addition, Infraction mass expressed as percentage of the left ventricle weight was found to be non-significantly changed between Anakinra treated group and Ischemic Control group; moreover, on comparing Apelin with Amlodipine treated groups, no significant changes were found. Conclusions: Administration of Apelin, Amlodipine and Anakinra prior to myocardial reperfusion reduces infarct size and markers of infarction in experimental ischemia/reperfusion injury with highest efficacy of Ana- kinra more than Apelin and Amlodipine. P534 / #319, E-POSTERS TOPIC: 4. CLINICAL VASCULAR DISEASE / 4.01 COAGULATION AND THROMBOSIS. ERYTROCYTES DEFORMABILITY IN PATIENTS WITH CORONARY ARTERY DISEASE AND ANEMIA N. Pavliukovych , O. Pavliukovych. Bukovinian State Medical University, Department Of Internal Medicine, Clinical Pharmacology And Occupational Diseases, Chernivtsi, Ukraine Background and Aims: Rheological disorders in patients with coronary artery disease (CAD) are of great importance as they lead to the disease progression. They are mainly conditioned by changes of the erythrocyte membrane as red blood cells compose up to 45% of the cells in the bloodstream. Aim of the investigation e to detect possible changes of the erythrocyte deformability index (EDI) in patients with CAD and comorbid anemia. Methods: Tannert C. і Lux W. filtration method was used to achieve the aim. Patients were randomized into two groups: I e patients with CAD (n¼12), II e patients with CAD and anemia (n¼82). Results: Analysis of the results of the investigation indicates statistically significant reduction of EDI in patients of II group compared with patients of I group (1,23±0,04 against 1,69±0,10, p<0,02). It was established that with an increase of the anemia severity erythrocytes deformability had been decreasing gradually. EDI was the lowest at severe degree of anemia (1,02±0,06), which is 40% less than in patients with CAD without anemia (p<0,01), as well as at 29 and 17% less than due to mild and moderate degrees of severity respectively (1,43±0,04 and 1,24±0,07, р<0,05). Conclusions: Therefore, deformation ability of the erythrocytes in patients with CAD is significantly lower in case of comorbid anemia. As the degree of anemia severity increases, index of erythrocyte deformability decreases gradually which is accompanied by aggravation of blood rheological dis- orders. P535 / #408, E-POSTERS TOPIC: 4. CLINICAL VASCULAR DISEASE / 4.01 COAGULATION AND THROMBOSIS. DEVELOPMENT FEATURES OF CORONAVIRUS DISEASE COMPLICATIONS IN PATIENTS WITH CORONARY HEART DISEASE N.M. Nurillaeva 1 , M.M. Makhkamova 1 , K.B. Omarov 2 . 1 Tashkent Medical Academy, Internal Disease N  1, Tashkent, Uzbekistan; 2 Multidisciplinary clinic of the Tashkent Medical Academy, Cardiology, Tashkent, Uzbekistan Background and Aims: To study the incidence and clinical features of myocardial infarction development in patients with coronary heart disease (CHD) after suffering coronavirus disease. Methods: We studied clinical condition of 64 patients admitted with chest pain complaints to the emergency room of the multidisciplinary clinic of the Tashkent Medical Academy. The average age was 52,5±2,4 years. Results: Only 41 patients (64%) had acute myocardial infarction (MI) with various localization. The group included 10 women and 31 men. The pain verbal scale showed a low level of pain syndrome, as well as an atypical location of pain in the heart. In 13 patients, was detected a high level of IgG of coronavirus infection, whereas IgM level was high in 28 patients. The level of fibrinogen in the blood plasma averaged 355 g/l, the blood sugar level was around 7.3 mmol/l on an empty stomach, hematocrit level increased to approximately 0.49 l/l. In men, was noted the decrease of blood hemoglobin to 101.6 g/l. The rest of the indicators were practically not deviated from the norm. The ECG shows typical elevations of the ST segment from the isoline. Conclusions: Atypical clinical development signs of MI in patients with CHD is associated with a previous coronavirus infection, a large amount of lung tissue damage, a low awareness of patients about possible compli- cations of CHD and low adherence to maintenance drug therapy after suffering Covid-19. P536 / #410, E-POSTERS TOPIC: 4. CLINICAL VASCULAR DISEASE / 4.01 COAGULATION AND THROMBOSIS. LEVEL OF THROMBIN ACTIVATED FIBRINOLYSIS INHIBITOR IN PATIENTS WITH ISCHEMIC STROKE N.M. Nurillaeva, M.B.Q. Zokirova . Tashkent Medical Academy, Internal Disease N  1, Tashkent, Uzbekistan Background and Aims: The purpose of the study to determine the rela- tionship of the thrombin-activated fibrinolysis inhibitor with coagulation and fibrinolysis parameters. Methods: A total of 115 patients with acute cerebrovascular accident and hypertension were examined in the intensive care unit. The average age of patients was 57.5±1.0 years, including 47 women (40.8%) and 68 men (59.1%). Of these, 74 patients were found to have an ischemic type of ce- rebral circulatory disorders according to the conclusion of instrumental research methods. Results: A thrombin-activated fibrinolysis inhibitor (TAFI) involved in regulating the balance between coagulation and fibrinolysis was found in 82 (71.3%) patients. The average TAFI concentration was 180±20.97 ng / ml. High plasma TAFI levels may contribute to a hypofibrinolytic condition and an increased risk of thrombotic disorders.The existence of TAFI explains the obvious profibrinolytic effect of activated protein C and suggests the presence of a clear molecular link between the blood clotting of fibrinolytic cascades, which is expressed through thrombin-thrombomodulin- dependent activation of TAFI. Conclusions: The formation of thrombin can lead to the suppression of fibrinolysis. P537 / #543, E-POSTERS TOPIC: 4. CLINICAL VASCULAR DISEASE / 4.01 COAGULATION AND THROMBOSIS. Abstracts / Atherosclerosis 331 (2021) e56ee293 e226