©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 2939-2948 (2014) Association of beta 2 adrenoceptor gene polymorphisms in Malaysian hypertensive subjects M. Komara 1 , R. Vasudevan 2 , P. Ismail 1 , S.A. Bakar 1 , S.R. Pishva 1 and F. Heidari 1 1 Genetic Research Group, Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia 2 Institute of Gerontology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Corresponding author: P. Ismail E-mail: patimahismail@gmail.com Genet. Mol. Res. 13 (2): 2939-2948 (2014) Received January 3, 2013 Accepted July 31, 2013 Published April 16, 2014 DOI http://dx.doi.org/10.4238/2014.April.16.2 ABSTRACT. The sympathetic nervous system plays a major role in blood pressure regulation. Beta 2 (β2) adrenoceptor gene polymorphisms have been associated with hypertension in different populations with conficting results. We examined the association of three common polymorphisms, Arg16Gly, Gln27Glu, and Thr164Ile, of the β2 adrenoceptor gene in Malaysian hypertensive subjects. A total of 160 hypertensive and control subjects were recruited. Systolic blood pressure (SBP), diastolic blood pressure (DBP), and anthropometric measurements were obtained from each subject. Biochemical analyses of lipid profles were conducted with an autoanalyzer. DNA samples were extracted from blood and buccal cells. Genotyping was accomplished with polymerase chain reaction-restriction fragment length polymorphism. SBP, DBP, body mass index, and biochemical factors all differed signifcantly between case and control subjects