International Journal of Science and Research (IJSR) ISSN: 2319-7064 SJIF (2022): 7.942 Volume 11 Issue 8, August 2022 www.ijsr.net Licensed Under Creative Commons Attribution CC BY Bio Adsorption Study of Tabernaemontana divaricata Leaves Ash Ankita Kulshreshtha 1 , Jyoti Saxena 2 1, 2 Department of Chemistry, Institute for Excellence in Higher Education, Kaliyasot Dam, Kolar road, Bhopal, India 1 Corresponding author Email: ankita.kuls[at]gmail.com Abstract: The application of activated leaf ash of T. divericata for the removal of methyl orange from aqueous solution was investigated in this study. Five dilutions of 10 μg/ml of synthetic dye methyl orange were prepared as adsorbate and 10 mg, 20 mg, 30 mg, 40 mg and 50 mg activated leaf ash of T. divericata as adsorbent used for bio adsorption study. The maximum bio-adsorption was found when 97 mg/100 ml methyl orange dye was absorbed by 50 mg amount of leaf ash adsorbent. This study concluded that T. divericata leaves, which are abundantly available, can be utilized as efficient bio adsorbent for the removal of methyl orange from waste water release from industries. Keywords: Tabernaemontana divaricata, Leaves, Methyl Orange, Adsorption activity, Bio adsorbent 1. Introduction In many countries industrial sector extensively uses synthetic dyes for coloring products, producing large quantities of colored wastewater through the different dyeing and finishing processes [1]. The removal of dyes from effluents of textile, paper, plastic and cosmetic industries is one of the active areas of pollution control research [2]. Various parts of different plants as bio adsorbents have been explored for their adsorption abilities towards methyl orange as use in many applications. Increasing research interest is being envisaged during the recent past, in exploring the adsorption potentialities bio-waste materials of flora and fauna origin in controlling the polluting ions. These bio- processes along with other chemical processes are proving to be potential alternative to the existing methods of detoxification and for the recovery of toxic and valuable ions from industrial discharges and polluted waters [3]. Available methods for the removal of dyes from textile effluent include coagulation-flocculation [4], electro chemical oxidation, biodegradation, ion exchange [5], ozonation [6], adsorption by activated carbon [7]. However, these methods do not result incomplete removal of dyes from the effluent. As mentioned, conventional methods of sewage treatment and other biological methods are not suitable for the treatment of dye effluents due to the inability of micro-organisms to break down these compounds. Progress in industrialization in particular textile industries have led to the discharge of unprecedented amount of wastewater containing synthetic dyes, which pollutes the rivers and consequently causes harm to human and other living organisms [8]. Most of the synthetic dyes are not biodegradable and toxic. Methyl orange (MO) is a commonly used monoazo dye in laboratory assays, textiles and other commercial products and has to be removed from water due to its toxicity [9-10]. A majority of the used dyes are azo reactive dyes which are bright in color due to the presence of one or several azo (─N═N─) groups associated with substituted aromatic structures [11]. These dyes or their breakdown products are toxic to living organisms [12]. Furthermore, dyes in wastewater are difficult to remove because they are stable to light, heat and oxidizing agents. In short, they are not easily degradable [13-14]. Physical adsorption techniques are generally considered as the preferred means for removing and purifying organic substances due to their high efficiency and ability to separate a wide range of chemical compounds [15-16]. Many types of adsorbents are available for the removal of dyes. Activated carbon is the most commonly used adsorbent for removal of a vast variety of dyes but is too expensive and difficult for regeneration [17]. Therefore, there are demands for alternative low-cost adsorbents in order to make the adsorption treatment technology. The past few years have seen the successful applications of spent tea leaves [18], moringa peregrina plant [19], activated papaya leaf [20], corn leaves [21], various fruit peels such as watermelon rinds [22], mango peel [23], pomegranate peel [24], banana peel [25], dragon fruit peel, leaf-based adsorbents [26], etc. for removing dyes from aqueous solution via adsorption process. This study concluded that T. divericata leaves, which are abundantly available, can be utilized as efficient bio adsorbent for the removal of methyl orange from waste water release from industries. 2. Methodology 2.1 Adsorbent Leaves of T. divericata plant from the family Apocenacy were collected from local area of Bhopal city of Madhya Pradesh, India and were washed with distilled water then dried in shed. Kept leaves at ambient temperature for 24 h and was ground using a high speed blender and sieved to isolate fibers of the size 212-350 micron. For carbonation, powder leaves were heated to 90 o C in hot air-oven for 6 hrs then washed with distilled water several times to make free Paper ID: SR22816201255 DOI: 10.21275/SR22816201255 984