Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Thu, 06 Dec 2018 04:36:35 Streptomyces burgazadensis sp. nov., isolated from soil Salih Saricaoglu, 1 Kamil Isik, 1 3 Aysel Veyisoglu, 1 Hayrettin Saygin, 1 Demet Cetin, 2 Kiymet Guven, 3 Cathrin Spro ¨ er, 4 Hans-Peter Klenk 4 and Nevzat Sahin 1 3 Correspondence Kamil Isik kamilis@omu.edu.tr Nevzat Sahin nsahin@omu.edu.tr 1 Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey 2 Science Teaching Programme, Gazi Faculty of Education, Gazi University, Ankara, Turkey 3 Anadolu University, Faculty of Science, Biology Department, 26470, Eskis ¸ ehir, Turkey 4 Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany A novel actinobacterial strain, designated Z1R7 T , was isolated from a soil sample collected from Burgazada, in the Marmara Sea (Turkey), and the strain identity was determined using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and it formed a distinct phyletic line in the 16S rRNA gene tree, together with the type strains Streptomyces specialis GW41-1564 T (95.76 %), Streptomyces mayteni YIM 60475 T (95.64 %), Streptomyces hainanensis YIM 47672 T (95.53 %), Streptomyces hoynatensis S1412 T (95.29 %), Streptomyces avicenniae MCCC 1A01535 T (94.74 %), Streptomyces sedi YIM 65188 T (94.59 %) and Streptomyces zhaozhouensis NEAU-LZS-5 T (94.68 %). Chomotaxonomic data revealed that strain Z1R7 T possesed MK-9 (H 8 ) as the predominant menaquinone, LL- diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose and ribose as whole cell sugars. Diphosphatidylglycerol, phoshphatidylethanolamine and phosphatidylinositol were the predominant polar lipids; iso-C 16 : 0 , anteiso-C 17 : 0 and anteiso-C 15 : 0 were the major fatty acids, and the genomic DNA G+C content was 69.4 mol%. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1R7 T (5KCTC 29434 T 5DSM 42126 T ) should be classified in the genus Streptomyces as Streptomyces burgazadensis sp. nov. The genus Streptomyces represents a group of micro- organisms that are widely distributed in nature, encom- passing nearly 630 species with validly published names (http://www.bacterio.net/index.html). The genus Strepto- myces was first introduced by Waksman & Henrici (1943) to accommodate aerobic, spore-forming actinomycetes. Members of the genus Streptomyces are able to form an extensively branched substrate mycelium and are also able to produce aerial hyphae that typically differentiate into chains of spores. Members of the genus have LL-diaminopimelic acid in the cell-wall peptidoglycan, but no characteristic sugars (Lechevalier & Lechevalier, 1970) and possesses DNA rich in G+C (Williams et al., 1983; Manfio et al., 2003). Species of the genus Streptomyces are abundant in soil and are well known for their ability to produce biologically active sec- ondary metabolites, including antibiotics, enzymes, enzyme inhibitors, antitumour agents and antifungal compounds (Be ´rdy, 2005; Goodfellow & Fiedler, 2010). In recent years, the search for novel pharmaceutical agents against drug- resistant microbes from common species of the genus Streptomyces has become a more difficult and costly task. Hence, more attention has been focused on isolating streptomycetes from unusual and unexplored environments, such as the oceans (Veyisoglu & Sahin, 2014) and desert soil (Santhanam et al., 2013). During an onging investigative project on the diversity of actinobacteria in Burgazada (40 u 529 520N 29 u 49 80E) in the Sea of Marmara, soil samples were collected from a Pinus brutia Ten. (Turkish pine) forest. Strain Z1R7 T was isolated after incubation for 3 weeks at 28 u C on modified Bennett’s agar (MBA; Jones, 1949) supplemented with filter-sterilized cycloheximide (50 mg ml 21 ), nalidixic acid 3These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Streptomyces burgazadensis Z1R7 T is KF793919. Three supplementary figures and three supplementary tables are available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2014), 64, 4043–4048 DOI 10.1099/ijs.0.065870-0 065870 G 2014 IUMS Printed in Great Britain 4043