Submit Manuscript | http://medcraveonline.com Introduction Drugs of Natural origin have been used since ancient times as medicines for treating range of diseases. Medicinal plants have played a key role in world health. In spite of the great advances observed in modern medicine in recent decades, plants still make an important contribution to health care. Each plant is like factory capable of synthesizing unlimited number of highly complex and unusual chemical substances whose structures could otherwise escape the imagination forever. 1 The extract of the medicinal plant may consist of several phytochemical constituents, which often act together synergistically/at times antagonistically. Formulations based on natural products have reached widespread acceptance as medicinal agents for plethora of diseases. 2 The acceptance or the popularity of herbal products is on the rise, one of the hinderance in its total acceptance is that somewhere the reproducible quality benchmark parameters are lacking, the reasons may be known or unknown , for example due to the highly complex nature and known nature of variation of the phytochemical of drugs of natural origin. Furthermore, the structure elucidation part is at times not fully understood or needs some more studies with reproducible results to reach a decisive conclusion. 3 To overcome these uprising problems, standardization of herbal formulations is essential in order to assess the quality of drugs. Herbal medicines may contain up to hundreds of compounds. Although many of them are present in low concentrations, they may be important for the quality, safety and effcacy of the herbal medicines as their therapeutic effects are based on synergetic interactions between numerous constituents. In order to explore the whole profle, chromatography studies combined with a suitable detection technique offers a powerful tool for isolation of the individual phytochemical and thereby developing a characteristic profle of the given plant sample. 4 In the traditional text of Indian system of medicine, i.e. Ayurveda, medicinal plants play a signifcant role in maintaining good health. It would not be wrong to state that there is a resurgence of use of herbal medicines throughout the world and the people are turning back to natural remedies. Herbal medicinal products are dietary supplements that people administer to improve their health and are sold as tablets, capsules, powders, teas, extracts and fresh or dried plants. The literature survey reveals that the many herbs are used in health product provide nutritional supplements help us to overcome the nutritional defciencies. It also helps us to boost our immune system. Nutritional supplements are also useful in getting rid of the toxins that are accumulated in our body. There are some of plants (Table 1) are commonly used in health products, and also reported in literatures provide as body weight gain. Gallic acid and β- Sitosterol are commonly constituents found in these plants, thus they are active constituent of polyherbal formulation used as health supplements. Gallic acid (GA, 3,4,5-trihydroxybenzoic acid), a plant phenol that occurs naturally and is present in Amla, Haritaki, Bhera , and, tea leaves, grapes, and other plants, both in its free state and as part of the tannin molecule. Gallic acid possesses cytotoxicity against cancer cells, anti-infammatory, antimutagenic, hepatoprotective, and neuroprotective effect, anti-tumor potential and analgesic activity. 13‒15 Pharm Pharmacol Int J. 2016;4(4):373‒379. 373 © 2016 Ranjana et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and build upon your work non-commercially. Determination of Gallic acid and β–sitosterol in poly-herbal formulation by HPTLC Volume 4 Issue 4 - 2016 Ranjana, 1 Anurag Mishra, 1 Ashutosh Mishra, 2 Rajiv Gupta 1 1 School of Pharmacy, BBD University, India 2 Proffesor and Principal, Acharya Narendra Dev College of Pharmacy, India Correspondence: Rajiv Gupta, Professor and Dean School of Pharmacy, BBD University, Lucknow-226 028 India, Tel +91 9839278227, Fax +91 0522 285187, Email rajiv961@rediffmail.com Received: October 30, 2014 | Published: June 07, 2016 Abstract Introduction: In recent years, herbal medicines are increasing in popularity all over the world. The use of herbal medicines are being used in both preventative and treatment based usage, as health supplements and tonics because consumers perceive herbals as “natural”, safe, harmless and free from adverse side effects. Aim: The present studies aims to quantitatively estimate Gallic acid and β-Sitosterol in selected Polyherbal formulations. Methods and Material: Polyherbal capsules available in market were randomly selected for studies. The Parameters like shape, size, colour, pH, weight variation, Moisture content disintegration time and dissolution time were studied. Chromatographic and HPTLC method was applied for the qualitative as well as quantitative determination of active constituent Gallic acid and β-Sitosterol. Result: The Gallic acid was found as 9.78µg/ mg and 12.79µg/ mg in S1and S2 respectively and β- sitosterol 4.18µg/ mg and 3.81µg/mg mg in S1and S2 respectively. Conclusion: It was thought worthwhile to initiate on generation of some data, for the evaluation of such types of Ayurvedic preparation i.e. capsules which may serve as a reference for future studies based on quality control. Keywords: β-sitosterol, gallic acid, HPTLC, fingerprint, polyherbal, capsules, weight gain Pharmacy & Pharmacology International Journal Research Article Open Access