Nanoparticle mediated CRISPR base editing rescues Kir7.1 function relevant to ocular channelopathy Authors: Meha Kabra 1, 2, † , Pawan K. Shahi 1, 2, † , Yuyuan Wang 3, 4 , Divya Sinha 2, 5 , Allison Spillane 1 , Gregory A. Newby 6, 7, 8 , Shivani Saxena 2, 3, 4 , Amr A. Abdeen 2, 3, 4 , Kimberly L. Edwards 2, 5 , Cole O. Theisen 2, 5 , David M. Gamm 2, 9 , David R. Liu 6, 7,8 , Shaoqin Gong 2, 3, 4, 9 , Krishanu Saha 1, 2, 3, 4, 10, * , Bikash R. Pattnaik 1,2,9, * Author affiliations: 1 Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, United States. 2 McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, United States. 3 Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States. 4 Wisconsin Institute of Discovery, University of Wisconsin-Madison, Madison, WI, United States. 5 Waisman Center, University of Wisconsin-Madison, Madison, WI, United States. 6 Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, United States. 7 Howard Hughes Medical Institute, Harvard University, Cambridge, MA, United States. 8 Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, United States. 9 Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI, United States. 10 Center for Human Genomics and Precision Medicine, University of Wisconsin-Madison, Madison, WI -, United States. † These authors contributed equally to this work. *Corresponding author: Email: pattnaik@wisc.edu and ksaha@wisc.edu (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted August 23, 2022. ; https://doi.org/10.1101/2022.07.12.499808 doi: bioRxiv preprint