Int.J.Curr.Microbiol.App.Sci (2018) 7(5): 912-916 912 Original Research Article https://doi.org/10.20546/ijcmas.2018.705.111 Study the Impact of Age on Enzyme and Hormone Profile of Crossbred Calves Milap G. Sedhiya 1 , M.M. Pathan 1* , A.M. Pande 1 , S.P. Madhira 1 , R.J. Modi 2 and N.P. Sarvaiya 3 1 Department of Veterinary Physiology and Biochemistry, College of Veterinary Sci. & A. H., AAU, Anand, Gujarat, India 2 Department of Livestock Research Station, AAU, Anand, Gujarat, India 3 RBRU, AAU, Anand, Gujarat, India *Corresponding author ABSTRACT Introduction The most vulnerable period in the life of any animal is the period after birth. In all species, the neonatal period represents a critical time during which all organ functions must adapt to the extra-uterine life; it is a transition phase from the sheltered intra-uterine to the exposed extra-uterine environment (Piccione et al., 2008). The increasing use of serum enzyme changes as aid to clinical diagnosis creates a need for understanding variations in serum enzymes in newborn animals. Marked temporary increase in serum activities of International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 05 (2018) Journal homepage: http://www.ijcmas.com This study was conducted to evaluate the impact of age on serum enzymes and hormones in crossbred calves from birth to three months of age. The research was carried out on six apparently healthy crossbred calves (aged 0-3 months) with similar physiological status. Blood samples were collected from the experimental animals on 0, 3, 7, 10, 15, 30, 45, 60, 75 and up to 90 days age of calves. Five ml of whole blood from each animal was collected in clot activator vacutainer. Serum was separated and analyzed for serum enzymes and hormones. The concentration of Alanine Transaminase and Aspartate Transaminase were significantly (P < 0.05) highest on the day of birth while significantly (P < 0.05) lowest concentration was observed on 90 th day of life. The serum Alkaline Phosphatase concentration was highest on the day of birth and decreased significantly (P < 0.05) on 3 rd day. The serum Triiodothyronine (T 3 ) and Thyroxine (T 4 ) levels were significantly (P < 0.05) highest on the day of birth. While the lowest concentration of T 3 and T 4 were observed on 90 th day of life. The level of T 3 and T 4 were decreasing significantly (P < 0.05) from the day of birth to 90 th day of life. The non-significant difference was observed for serum cortisol from the day of birth to 90 th day of life in crossbred calf. It could be concluded that among obtained among enzymes, ALT, AST and AKP having significant variation. Triiodothyronine and throxine levels differed significantly while level of cortisol was not affected significantly during pre-weaning period of crossbred calf. Keywords Enzymes, Hormones, Crossbred calves Accepted: 10 April 2018 Available Online: 10 May 2018 Article Info