INTERNATİONAL JOURNAL OF AGRİCULTURE & BİOLOGY
ISSN Print: 1560–8530; ISSN Online: 1814–9596
16–251/2016/18–5–1075–1080
DOI: 10.17957/IJAB/15.0211
http://www.fspublishers.org
Full Length Article
To cite this paper: Kaya, M.D., E.G. Kulan, H. Dağhan, O. İleri and S. Avcı, 2016. Efficiency of vigor tests and seed elemental concentrations to estimate
field emergence in soybean (Glycine max). Int. J. Agric. Biol., 18: 1075‒1080
Efficiency of Vigor Tests and Seed Elemental Concentrations to Estimate
Field Emergence in Soybean (Glycine max)
Mehmet Demir Kaya
1*
, Engin Gökhan Kulan
1
, Hatice Dağhan
2
, Onur İleri
1
and Süleyman Avcı
1
1
Department of Field Crops, Eskişehir Osmangazi University, Eskişehir, Turkey
2
Department of Soil Sciences and Plant Nutrition, Eskişehir Osmangazi University, Eskişehir, Turkey
*
For correspondence: demirkaya76@hotmail.com
Abstract
The relationship between seed vigor test and seedling emergence is crucial to predict easily the seed performance under field
conditions and the seed producers continuously explore the best vigor test to enhance stand establishment. This study was
conducted to determine the suitable seed vigor test and mineral element in the seed to predict accurately the field performance
of soybean. Fifty seed lots of 22 commercial soybean cultivars were assessed for seedling emergence under field conditions
and germination, emergence, cool and cold tests, electrical conductivity (EC) and pH for 4, 8 and 24 h, accelerated ageing
(AA) at 41, 43 and 45°C for 48, 72, 96, 120 and 144 h in the laboratory. Initial seed weight, oil, protein, P, K, Ca, Fe, Mn and
Zn concentration in seed lots were also investigated. Their results were correlated with field emergence percentage of seed lots
to detect the most suitable indicator for vigorous seed lot. The germination percentage varied from 56% to 100%, while the
field emergence percentage ranged between 0% and 84%. No significant correlation was noticed between field emergence and
seed weight, oil and protein contents. AA test at 41°C for 72 h gave the highest significantly positive correlation with
r=0.831**, but the highest significant and negative correlation coefficient (r=-0.801**) with field emergence percentage were
observed in EC test for 24 h. Only Mn concentration of six elements investigated was significantly correlated with field
emergence (r=-0.376*). It was concluded that the AA condition of 72 h at 41°C are recommended as an ideal seed vigor test to
estimate closely field emergence performance of soybean seeds. © 2016 Friends Science Publishers
Keywords: Glycine max L.; Germination; Seedling emergence; Accelerated ageing; Seed quality
Introduction
Soybean (Glycine max L.) is an economically important
protein rich oilseed crop with variety of uses, including wide
use in poultry industry of Turkey. Its production is
supported by the Turkish government to avoid import of
soybean for local uses (Kolsarıcı et al., 2015; TUIK, 2016).
It has been extensively cultivated as a main and second crop
after winter wheat or barley in the Mediterranean region of
Turkey (Çopur et al., 2009). The difference between
planting dates lead to exposing the seeds to adverse
environmental conditions, namely, water lodging, low
temperature and crust formation in the main crop, or high
soil temperature, drought and poor seedbed preparation in
double-crop planting (Khaliliaqdam et al., 2013). For these
reasons, the vigorous seed production is obligatory for
regular germination, seedling emergence and a good stand
establishment.
Seed suppliers consider the standard germination test
as an evidence of seed quality; that is conducted under
optimal conditions in terms of temperature and moisture for
germination. It doesn't completely estimate the seedling
emergence performance because field conditions are rarely
optimum for germination, which adversely affect the
emergence and subsequent seedling growth (Hampton and
TeKrony, 1995; Kolasinska et al., 2000; Khan et al., 2010).
Tekrony and Egli (1977) reported the standard germination
exhibited a significant correlation with field emergence of
soybean only if favorable field conditions have been
available while Makkawi et al. (1999) demonstrated the
standard germination was the only test to be useful for
predicting field emergence of lentil. Usha and Dadlani
(2015) found that germination test was the worst test
predicting field performance of soybean among the
investigated vigor tests.
Several seed vigor tests have been developed for better
prediction of field performance of seed lots (Hampton and
Coolbear, 1990; ISTA, 2002; Miloševic et al., 2010; Arif et
al., 2014). Colete et al. (2004) and Vieira et al. (2004)
reported that electrical conductivity (EC) may be efficient to
determine seed vigor in soybean while Salinas et al. (2010)
the minimum 19 hours must require for the stable EC results
in soybean. Marcos-Filho et al. (2001) found the accelerated
ageing and controlled deterioration tests could be used to
select vigorous seed lots in soybean. Vieira et al. (2010)
suggests that the cold test conducted between papers at