Research Article
Biochemical and Histological Evaluation of Three Selected
Medicinal Plant Extracts of Sri Lankan Origin on Dyslipidemia
and Oxidative Stress in Alloxan Induced Diabetic Rats
Anoja Priyadarshani Attanayake ,
1
Kamani Ayoma Perera Wijewardana Jayatilaka ,
1
Lakmini Kumari Boralugoda Mudduwa,
2
and Chitra Pathirana
1
1
Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Karapitiya, Sri Lanka
2
Department of Pathology, Faculty of Medicine, University of Ruhuna, Karapitiya, Sri Lanka
Correspondence should be addressed to Anoja Priyadarshani Attanayake; anoja715@yahoo.com
Received 26 January 2018; Accepted 17 May 2018; Published 12 June 2018
Academic Editor: Tariq Mahmood
Copyright © 2018 Anoja Priyadarshani Attanayake et al. Tis is an open access article distributed under the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
Te objective of the present study was to investigate the efect of refuxed aqueous extracts of Gmelina arborea, Spondias pinnata,
and Coccinia grandis on atherogenicity and oxidative stress in rats with chemically induced type 1 diabetes mellitus. Alloxan
monohydrate (150 mg/kg, intraperitoneal) was used to induce diabetes to Wistar rats. Tereafer, diabetic rats (n=6 per group)
were treated with the three selected plant extracts at their optimum efective therapeutic doses and glibenclamide (0.50 mg/kg,
positive control) for 30 days. Administration of the three extracts in diabetic rats exhibited antihyperglycemic, antiatherogenic,
and antioxidant efects in diabetic rats on the 30th day of the study. Te atherogenic and coronary risk indices were also reduced in
support of the antiatherogenic efects. Te results of the study revealed that the bark extracts of G. arborea, S. pinnata, and leaf extract
of C. grandis exerted benefcial efects against dyslipidemia, atherogenicity, and oxidative stress in alloxan induced diabetic rats. Te
selected plant extracts would be benefcial for the development of food supplements targeting main complications associated with
diabetes.
1. Introduction
Diabetes mellitus (DM) is a leading cause of morbidity and
mortality worldwide and predicted to afect over 500 million
people by 2030. Indeed, it is a major cause of disability and
hospitalization, posing a signifcant public health burden
during the last two decades [1, 2].
Te chronic hyperglycemia of diabetes is associated
with deleterious damage, dysfunction, and failure of various
organs. Oxidative stress has been suggested as a main contrib-
utory factor in the pathogenesis of diabetes and its vascular
complications leading to neuropathy, retinopathy, nephropa-
thy, cardiovascular disease, etc. [3]. In addition, diabetes
is closely associated with dyslipidemia which is mediated
through derangements in a variety of regulatory processes,
especially in a state of insulin defciency, thereby render-
ing diabetic patients more prone to hypercholesterolemia
and hypertriglyceridemia. Several studies have revealed the
positive correlation between dyslipidemia and development
of premature atherosclerosis, coronary insufciency, and
myocardial infarction in diabetic subjects [4–7]. However, the
occurrence of vascular complications in patients with dia-
betes mellitus has not been met with a comparable expansion
in therapeutic options [1]. Tus, efect of natural products
in the management of diabetic complications has recently
received considerable attention, highlighting the importance
of medicinal plant extracts as regulators of metabolism of
carbohydrate and lipids [8].
It is being appreciated that traditional systems of
medicine can ofer some efective therapies from their trea-
tise to be useful in diabetic complications. Te remarkable
chemical diversity encompassed by the medicinal plant
extracts continues to be of relevance to the discovery of new
antidiabetic agents with fewer side efects [9]. A number of
Hindawi
Journal of Botany
Volume 2018, Article ID 4204519, 8 pages
https://doi.org/10.1155/2018/4204519