J I T A A Journal of the Indonesian Tropical Animal Agriculture Accredited by Ditjen Penguatan Risbang No. 60/E/KPT/2016 J. Indonesian Trop. Anim. Agric. pISSN 2087-8273 eISSN 2460-6278 http://ejournal.undip.ac.id/index.php/jitaa 44(4):382-391, December 2019 DOI: 10.14710/jitaa.44.4.382-391 Fat deposition of broiler chickens fed a high-fat diet contained Sauropus androgynus leaf extract plus turmeric powder K. Kususiyah 1,* , U. Santoso 1 , Y. Fenita 1 , A. M. H. Putranto 2 and S. Suharyanto 1 1 Department of Animal Science, Faculty of Agriculture, Bengkulu University, Kota Bengkulu 38371A - Indonesia. 2 Department of Chemistry, Faculty of Mathetamics and Natural Science, Bengkulu University, Kota Bengkulu 38371A, Indonesia. * Corresponding E-mail: kususiyah@unib.ac.id Received July 02, 2019; Accepted November 04, 2019 ABSTRAK Rancangan acak lengkap faktorial digunakan untuk menganalisis pengaruh ekstrak daun katuk (EDK) plus tepung kunyit (TK) terhadap deposisi lemak pada ayam broiler yang diberi pakan tinggi lemak. Seratus lima puluh ayam broiler berumur 21 hari digunakan dalam penelitian ini. Faktor pertama adalah sumber lemak (6% lemak sapi dan 6% minyak sawit), dan faktor kedua adalah EDK plus TK [0 g EDK plus 0 g TK (G1), 9 g EDK plus 0,5 g TK (G2), 18 g EDK plus 0,5 g TK (G3), 9 g EDK plus 1 g TK (G4), 18 g EDK plus 1 g TK (G5)]. Suplementasi EDK plus TK mempengaruhi kolesterol, asam laurat, asam miristat, asam palmitat, asam stearat dan asam eikosapentaenoat daging (p <0,01). Sumber lemak mempengaruhi lemak, kolesterol, asam laurat, asam miristat, asam palmitat, asam stearat dan asam eikosapentaenoat daging (p <0,01). Terdapat interaksi yang signifikan antara dua faktor pada kadar lemak, kolesterol, asam laurat, asam palmitat, asam stearat, dan asam eicosapentaenoat daging. Kesimpulannya, suplementasi 18 g EDK plus 1 g TK pada pakan berlemak tinggi menghasilkan asam stearat yang lebih rendah, tetapi menghasilkan asam eikosapentaenoat yang lebih tinggi. Suplementasi EDK plus TK pada pakan tinggi lemak menurunkan kadar kolesterol daging dan mengubah komposisi asam lemak. Kata kunci: Sauropus androgynus, tepung kunyit, penimbunan lemak, asam lemak, broiler ABSTRACT A factorial design was used to analyze the influence of Sauropus androgynus leaf extract (SALE) and turmeric powder (TP) on fat deposition in broilers fed high-fat diet. The first factor was the source of fat (6% beef fat and 6% palm oil), and the second factor was SALE plus TP [0 g SALE plus 0 g TP (G1), 9 g SALE plus 0.5 g TP (G2), 18 g SALE plus 0.5 g TP (G3), 9 g SALE plus 1 g TP (G4), 18 g SALE plus 1 g TP (G5)]. SALE plus TP affected cholesterol, lauric acid, myristic acid, palmitic acid, stearic acid and eicosapentaenoic acid contents (p<0.01). Fat sources affected fat, cholesterol, lauric acid, myristic acid, palmitic acid, stearic acid and eicosapentaenoic acid (p<0.01). There was a significant interaction between the two factors on fat, cholesterol, lauric acid, palmitic acid, stearic acid, and eicosapentaenoic acid contents. In conclusion, 18 g SALE plus 1 g TP supplementation to high-fat diet resulted in lower stearic acid, but it resulted in higher eicosapentaenoic acid. Supplementation of SALE plus TP to a high-fat diet lowered cholesterol content and changed fatty acids composition. Keywords: Sauropus androgynus,turmeric powder,fat deposition,fatty acid, broilers 382 J.Indonesian Trop.Anim.Agric. 44(4):382-391, December 2019