Borneo Journal of Pharmacy http://journal.umpalangkaraya.ac.id/index.php/bjop/article/view/1916 Vol 4 Issue 3 August 2021 DOI: https://doi.org/10.33084/bjop.v4i3.1916 Page 171 – 177 e-ISSN: 2621-4814 INTRODUCTION Asthma is a disease with an increasing prevalence rate, and there is a strong interaction between airway epithelial cells and the immune system against the pathogenesis of asthma 1 . The most common immunopathology in asthma is inflammation of type 2 characterized by processes in the airway epithelium involving cytokines 2 . Symptoms of asthma include wheezing, shortness of breath, heavy chest, and coughing at night and early morning. There is muscle contraction in the bronchial walls, swelling of the bronchial mucosa, and increased mucus production in the secretory cells of the bronchial mucosa, resulting in increased resistance to airflow through the narrowed bronchi. This condition occurs in shortness of breath 3,4 . The increase in the eosinophil count in asthmatic patients correlated with the severity of the respiratory failure and was predicted to increase the risk of sequential sub- exacerbations. Immunoglobulin E (IgE) is involved early in the inflammatory cascade and may be considered a cause of allergic asthma 5,6 . Moringa oleifera Lam. is considered a miracle plant with many benefits for humans and is credible for providing good nutrition and healing and preventing many diseases 7 . The pharmacologically reported effects include antibacterial, antifungal, antiviral, anti-inflammatory and analgesic, antioxidant, hypotensive, antiulcer, cardioprotective anesthetic, antiurolithic, and wound healing activity 8-12 . The M. oleifera plant has various therapeutic properties. For example, the general nutritional content of M. oleifera to several specific properties, including anti-inflammatory, antimicrobial, Antiasthma Activities of Moringa oleifera Lam. Leaves Extract on the Eosinophil Count and Mast Cells in BALB/c Mice Dian Arsanti Palupi 1* Tri Wahyuni Prasetyowati 1 Dwi Murtiningsih 1 Dede Mahdiyah 2 1 Department of Pharmacy, STIKES Cendekia Utama Kudus, Kudus, Central Java, Indonesia 2 Department of Pharmacy, Universitas Sari Mulia, Banjarmasin, South Kalimantan, Indonesia * email: arsanti_palupi@yahoo.com Keywords: Asthma Eosinophil Moringa oleifera Abstract Asthma is a chronic inflammation of the respiratory tract. This study aimed to prove Moringa oleifera Lam. leaves extract's effect on reducing the eosinophil count and mast cells in asthmatic mice. Twenty BALB/c mice that met the inclusion criteria were divided into four groups of standard control (K1), negative control (K2), dexamethasone (K3), and M. oleifera leaves extract (K4). On days 0 and 7, intraperitoneal ovalbumin (OVA) was sensitized. On days 14, 16, and 18, mice were re-sensitized by inhalation using 1% OVA in sterile 0.9% NaCl. On days 18 to 25, K1 and K2 groups were given Na-CMC, the K3 group was given dexamethasone 1.3 µg/day, and the K4 group was given M. oleifera leaves extract 3.9 mg/day. On the 25 th day, the mice were terminated to analyze the eosinophil count and stable bronchiolar mast cells. In conclusion, M. oleifera leaves extract was proven to decrease the eosinophil count with a p-value <0.05 and could stabilize bronchiolar mast cells with a p-value <0.05. Received: December 11 th , 2020 Accepted: May 22 nd , 2021 Published: August 30 th , 2021 © 2021 Dian Arsanti Palupi, Tri Wahyuni Prasetyowati, Dwi Murtiningsih, Dede Mahdiyah. Published by Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya. This is an Open Access article under the CC-BY-SA License (http://creativecommons.org/licenses/by-sa/4.0/). DOI: https://doi.org/10.33084/bjop.v4i3.1916 Research Article