This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/jms.4622 This article is protected by copyright. All rights reserved. BAKIRDERE Sezgin (Orcid ID: 0000-0001-9746-3682) A novel and rapid extraction protocol for sensitive and accurate determination of prochloraz in orange juice samples: Vortex assisted spraying based fine droplet formation liquid phase microextraction before gas chromatography-mass spectrometry Yaren Dikmen a , Aybüke Güleryüz a , Berfin Metin a , Süleyman Bodur a , Miray Öner a , Sezgin Bakırdere a,b * a Yıldız Technical University, Faculty of Art and Science, Department of Chemistry, 34220 Davutpasa, Esenler, İstanbul, Turkey b Turkish Academy of Sciences (TÜBA), Piyade Street, No: 27, Çankaya, 06690, Ankara, Turkey ABSTRACT A novel, eco-friendly and easy extraction and preconcentration method named as vortex assisted spraying based fine droplet formation liquid phase microextraction was proposed for the determination of prochloraz at trace levels in orange juice samples by GC-MS. In this novel system, extraction solvent is dispersed by the help of spraying apparatus instead of dispersive solvent. Various parameters of the method were carefully optimized to increase signal to noise ratio of the analyte. Under the optimum chromatographic and extraction conditions, limit of detection and quantification were calculated as 3.2 and 10.8 μg/kg, respectively. Moreover, enhancement in quantification power for the GC-MS system was determined as 372 folds based on LOQ comparison. Relative recovery results for orange juice