Abstracts / Toxicology Letters 238S (2015) S56–S383 S85 repeatedly (15 mg, 30 mg/kg body wt./day) through oral route. The study was carried out on the basis of cytomorphological alterations of blood cells, bone marrow cells and numerical level of differ- ent blood indices following standard haematological techniques. Findings of the present study revealed an increase in TLC with abnormal count of DLC associated with gradual reduction in TLC and Hb content with alterations of its related indices in chem- ical treated groups. Cytomorphological changes of RBC showed transformations of numerous erythrocytes into stomatocytes with central slit-like pallor, tear drop cells and Heinze bodies, hyper- chromic microcytes and crenation of RBC membrane. A number of immature leucocytes viz. ring shaped eosinophil, granulocytic blast formation with fragmented neutrophil and irregular shaped bas- ket cells were evident in blood film with depleted haematopoesis in bone marrow. These chemical induced haematological changes were scavenged or became almost normal in animals fed with BS extract after withdrawal of chemical treatment. Analysis of the result concluded that BS extract has the potential to counteract the action of ETP induced haematotoxic effects. Hence BS extract is a potent haemato-protective agent. Funding Source: This work was supported by a grant no. [F-5-106/2013-14/(MRP/NERO)/592 dt. 23 May 2014] from the Uni- versity Grants Commission; New Delhi; India under the scheme of faculty improvement program. Disclosure statement: None of the authors have any conflicts of financial interest to declare. http://dx.doi.org/10.1016/j.toxlet.2015.08.284 P02-061 Re-evaluation of food colours by the European Food Safety Authority (EFSA) F. Lodi * , A. Tard, S. Tasiopoulou, P. Colombo, C. Roncancio Pe˜ na European Food Safety Authority, Food Ingredients and Packaging Unit, Parma, Italy Food additives are evaluated by the European Food Safety Authority Panel on Food Additives and Nutrient Sources added to food (ANS) with the support of the Food Ingredient and Pack- aging (FIP) Unit. Commission Regulation (EC) No. 257/2010 has set up a programme for the re-evaluation of approved food addi- tives (which were authorised before January 2009) in accordance with Article 32 of the Regulation (EC) No. 1333/2008 on food additives. Priorities have been set taking as a basis certain crite- ria such as the time since the last evaluation of a food additive was done by the Scientific Committee of Food (SCF) or by EFSA, the availability of new scientific evidence, the extent of use of a food additive in food and the human exposure to the food addi- tive. The re-evaluation of food colours has already been started with highest priority since these food additives have the oldest evaluations by the SCF, the deadline for the last food colour to be re-assessed being December 2015. To date, the remaining food colours to be re-evaluated under the re-evaluation programme are 12, including among others chlorophylls (E140(i)), chlorophyllins (E140(ii)) and copper complexes of chlorophylls and chlorophyllins (E141(i,ii)). Since some of the food colours already re-evaluated by EFSA (including the so-called “Southampton colours”) showed a possible exceedance of the Acceptable Daily Intake (ADI), for these food colours in parallel with the re-evaluation programme as laid down in the Regulation, EFSA has been asked to produce statements focused on the refined exposure. For a total of 10 colours according to four different priorities as provided by the European Commis- sion, refined exposure assessments have been performed. The main results indicates that, for the majority of those, with the additional information on their uses in foods as consumed, new consump- tion data and new methodology applied, the anticipated dietary exposures are considerably lower than in the previous exposure assessments and no longer exceed the ADI. These refinements are referring among others to caramel colours (E150 a,c,d), curcumin (E100), amaranth (E123), brown HT (E155), azorubine/carmoisine (E122) and allura red AC (E129). Results of the refinement of expo- sure for selected food colours and some peculiar features of other food colours are shown and discussed. http://dx.doi.org/10.1016/j.toxlet.2015.08.285 P02-062 Detection and quantification of 41 antibiotic residues’ in Gilthead sea bream (Sparus aurata) from aquaculture origin, using a multiclass and multi-residue UHPLC-MS/MS method L. Santos 1,2,* , B. Soares 1,2 , J. Rosa 1,2 , A. Freitas 1,2 , S. Leston 3 , J. Barbosa 3 , F. Ramos 3 1 Faculty of Pharmacy – University of Coimbra, Bromatology, Coimbra, Portugal 2 University, Pharmacy, Coimbra, Portugal 3 University, 2CFE – Centre for Functional Ecology, Department of Life Sciences, Coimbra, Portugal Aquaculture is one of the worldwide strategic development fields, and its importance is evident in its significant worldwide growth in the last decades. This growth is associated with the implementation of intensive and semi-intensive production meth- ods, with the use of antibiotics in order to prevent the emergence and spread of infectious diseases in fish. This practice constitutes a real public health problem, not only due to the presence of antimicrobial residues in edible tissues, which can cause allergic reactions in hypersensitive individuals, but also due to the emer- gence of bacterial resistance. Consequently, the European Union’s Regulatory Agencies have established maximum residue limits and specific requirements concerning the performance of analytical methods and the interpretation of the results. Nowadays liquid chromatography coupled with mass spectrometric detection is used as preferential tool. However, the current analytical strategy is shifting towards multi-residue and multiclass methods, which save time because all the target antibiotics are analyzed in the same run. At the present, such methods are relatively uncommon but we think they represent the future trend in this field. In the present study, an analytical methodology is being used for the simultane- ous determination of 41 antibiotics from seven different classes – sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol – in 27 samples of Gilthead sea bream collected in a number of supermarkets from North to South of Portugal. The method is based on UHPLC-MS/MS and has been fully validated in accordance with Decision 2002/657/EC, provid- ing evidence that it is suitable for application in routine analysis. The laboratorial work is being developed at the moment, and the authors estimate to have final results by May–June 2015. Acknowledgements: This study was funded by FEDER through the Operational Program for Competitiveness Factors – COMPETE, through FCT – the Portuguese Foundation for Science and Technol- ogy – under the Project PTDC/AGR/ALI/122119/2010. http://dx.doi.org/10.1016/j.toxlet.2015.08.286