The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggesstions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA, 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any oenalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. a. REPORT Analysis of redox responses during TNT transformation by Clostridium acetobutylicum ATCC 824 and mutants exhibiting altered metabolism 14. ABSTRACT 16. SECURITY CLASSIFICATION OF: The transformation of trinitrotoluene (TNT) by several mutant strains of Clostridium acetobutylicum has been examined to analyze the maximal rate of initial transformation, determine the effects of metabolic mutations of the host on transformation rate and to assess the cell metabolic changes brought about during TNT transfromation. Little difference in the maximal rate of TNT degradation in early acid phase cultures was found between the parental ATCC824 strain and strains altered in the acid forming pathways (phosphotransacetylase, or butyrate 1. REPORT DATE (DD-MM-YYYY) 4. TITLE AND SUBTITLE 13. SUPPLEMENTARY NOTES The views, opinions and/or findings contained in this report are those of the author(s) and should not contrued as an official Department of the Army position, policy or decision, unless so designated by other documentation. 12. DISTRIBUTION AVAILIBILITY STATEMENT Approved for public release; distribution is unlimited. UU 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 6. AUTHORS 7. PERFORMING ORGANIZATION NAMES AND ADDRESSES U.S. Army Research Office P.O. Box 12211 Research Triangle Park, NC 27709-2211 15. SUBJECT TERMS electron mediators, metabolism, redox, anaerobe, hydrogenase Matthew Servinsky, Xianpeng Cai, James Kiel, Christian Sund, George N. Bennett William Marsh Rice University Office of Sponsored Research William Marsh Rice University Houston, TX 77005 - REPORT DOCUMENTATION PAGE b. ABSTRACT UU c. THIS PAGE UU 2. REPORT TYPE New Reprint 17. LIMITATION OF ABSTRACT UU 15. NUMBER OF PAGES 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 5c. PROGRAM ELEMENT NUMBER 5b. GRANT NUMBER 5a. CONTRACT NUMBER W911NF-09-1-0119 611103 Form Approved OMB NO. 0704-0188 55744-LS.1 11. SPONSOR/MONITOR'S REPORT NUMBER(S) 10. SPONSOR/MONITOR'S ACRONYM(S) ARO 8. PERFORMING ORGANIZATION REPORT NUMBER 19a. NAME OF RESPONSIBLE PERSON 19b. TELEPHONE NUMBER George Bennett 713-348-4920 3. DATES COVERED (From - To) Standard Form 298 (Rev 8/98) Prescribed by ANSI Std. Z39.18 -