African Journal of Biology and Medical Research ISSN: 2689-534X Volume 4, Issue 3, 2021 (pp. 1-8) 1 Article DOI: 10.52589/AJBMR-DF4A8VSK DOI URL: https://doi.org/10.52589/AJBMR-DF4A8VSK www.abjournals.org INVESTIGATING THE EFFICACY OF POTENTIAL DIET-DERIVED PHYTOCHEMICALS ON LNCaP CELL LINES UNDER PHYSIOLOGICAL CONDITIONS Onuoha Chinyere 1 , Chukwudoruo C.S. 2 , Ezenwaka C.O. 3 and Ujowundu F.N. 4 1 Department of Biochemistry, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria E-mail: chizyonu@gmail.com 2 Department of Biochemistry, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria Email: cchukwudoruo@gmail.com 3 Department of Biology, Faculty of Science, Federal University Otuoke, Bayelsa State, Nigeria. Email: chyladyn@yahoo.com 4 Department of Biochemistry, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria Email: ujowundufn@gmail.com ABSTRACT: Prostate cancer (PCa) is increasingly prevalent in Nigeria and chemoprevention has a potential role to stem the initiation, promotion and progression of prostate carcinogenesis. The in vivo efficacy of phytochemicals depends on bioavailability, as the concentration of the compounds reaching target sites are known to reduce tremendously. With lower concentrations being achieved in target organs, the outcome on key players of carcinogenesis needs to be understood. The effect of curcumin, 3,3’-Diindolylmethane and epigallocatechin-3-gallate were interrogated on LNCaP cell lines by imitating in vivo conditions. The LNCaP cell lines were firstly, treated with low doses of curcumin, DIM and EGCG respectively. Subsequently, to investigate chemopreventive potentials of selected phytochemicals, cell lines were pre-treated and subsequently stimulated with testosterone. Lastly, to investigate the therapeutic potential of selected phytochemicals, cell lines were pre-stimulated before receiving respective treatments. From results, the effect of low dose treatments on the androgen receptor (AR) was dose-dependent. AR inhibition was observed more with cell lines that received a pre- treatment than cell-lines that received a pre-stimulation. All findings indicate that the investigated phytochemicals are potential chemopreventive regimens than curative regimens, since inhibitory effects on AR were enhanced more with a pre-treatment than a pre- stimulation. Furthermore, increased bioavailability of chemopreventive regimens will enhance efficacy. KEYWORDS: PCa, LNCaP, Androgen Receptor, Bioavailability, Chemoprevention Cite this article: Onuoha C., Chukwudoruo C.S., Ezenwaka C.O., Ujowundu F.N. (2021), Investigating the Efficacy of Potential Diet-Derived Phytochemicals on LNCaP Cell Lines Under Physiological Conditions. African Journal of Biology and Medical Research 4(3), 1- 8. DOI: 10.52589/AJBMR- DF4A8VSK. Manuscript History Received: 1 May 2021 Accepted: 21 May 2021 Published: 2 June 2021 Copyright © 2020 The Author(s). This is an Open Access article distributed under the terms of Creative Commons Attribution- NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), which permits anyone to share, use, reproduce and redistribute in any medium, provided the original author and source are credited.