*Correspondent: Fax: +90 242 227 4564; email: ngokoglu@hotmail.com Short communication Seasonal variation in fat content of anchovy (Engraulis encrasicolus) Nalan Göko` glu* 1 , Özkan Özden 2 , Nuray Erkan 2 , Taçnur Baygar 2 & Sühendan Metin 2 *1 University of Mediterranean, Faculty of Agriculture, Department of Food Engineering, Antalya, Turkey 2 Istanbul University, Fisheries Faculty, Istanbul, Turkey (Received 8 January 1999; Accepted in revised form 6 May 1999) Keywords Anchovy, fat content, seasonal variation. Introduction Anchovy (Engraulis encrasicolus) is a pelagic fish belonging to the Engraulidae family. It lives in areas from inshore to offshore, and is caught in depths of 100–180 m in the Mediterranean Sea and 60–70 m in the Black Sea. The species feeds mainly in summer months when water tempera- ture is 17–18 °C. In general, it migrates to coastal regions when the water temperature increases to reproduce and feed until October and November. Anchovy is the fish most frequently consumed in Turkey. Total landings were 290 680 t in 1996 and accounted for more than 50% of total fish production of 521 656 t of Turkey in that year (FAO, 1998). A significant portion (60%) of this harvest is utilized for human consumption and the remainder is processed into by-products (Acara et al., 1995). The oil content of the flesh of pelagic fish species varies in a regular annual cycle. The range may be very large from 1% to more than 25% wet weight (Sikorski et al., 1989; Burt & Hardy, 1992). The purpose of this paper was to investigate the seasonal variation of the fat content of anchovy caught in the Marmara Sea off Turkey. Materials and methods Raw material Samples of anchovy from the Marmara Sea were obtained through a local fish market in Ïstanbul twice each month from January to April and from September to December 1996. Fishing for anchovy from 15th of April 1996 to 1st September 1996 was prohibited in Turkey and samples could not be collected during that season. Approximately 1 kg fish, with an average length of 11 cm, were randomly selected for each sam- pling. The fish were gutted, beheaded, filleted and immediately analyzed. Analyses The fillets were homogenized using a blender and assays were carried out triplicate on samples of the homogenates. Moisture content was deter- mined by drying a sample in an oven at 105 °C to a constant weight (AOAC, 1990). Fat content was determined by method 948.15 described in AOAC (1990). The results are given as means of triplicate. Data were analyzed by analysis of vari- ance. Significance of differences between means were determined by Least Significant Difference. A significance level of 0.05 was chosen. Results and discussion Table 1 and 2 show the results of fat and mois- International Journal of Food Science and Technology 1999, 34, 401–402 © 1999 Blackwell Science Ltd 401