Remedy Publications LLC.
Annals of Pharmacology and Pharmaceutics
2017 | Volume 2 | Issue 10 | Article 1057 1
Tripartite Motif Proteins - A Protein Family Strongly
Linked to Cancer
OPEN ACCESS
*Correspondence:
Christine Blattner, Institute of Toxicology
and Genetics, Karlsruhe Institute of
Technology, Germany, Fax: +49 (0)
721-608 23354; Tel: +49 (0) 721-608
22634;
E-mail: christine.blattner@kit.edu
Received Date: 21 Mar 2017
Accepted Date: 30 May 2017
Published Date: 01 Jun 2017
Citation:
Elabd S, Andrew C. B. Cato, Christine
Blattner. Tripartite Motif Proteins - A
Protein Family Strongly Linked to
Cancer. Ann Pharmacol Pharm. 2017;
2(10): 1057.
Copyright © 2017 Christine
Blattner. This is an open access
article distributed under the Creative
Commons Attribution License, which
permits unrestricted use, distribution,
and reproduction in any medium,
provided the original work is properly
cited.
Short Communication
Published: 01 Jun, 2017
Short Communication
Cancer is a highly prevalent genetic disease and it is estimated that almost every second
person will sufer from the disease during his or her life-time. Despite intensive research over the
last 50 years, the development and progression of the disease is, in many cases, still incompletely
understood. In the past few years, a distinctive family of proteins called the TRIM (tripartite motif),
or RBCC (RING, B-box, coiled-coil region) protein family came into the focus of cancer research.
Te TRIM protein family is a very large family of proteins with more than 70 members in humans.
All proteins of this family have a tripartite motif in their N-terminus consisting of a RING (really
interesting new gene) domain, one or two B-boxes and a coiled-coil region. Te RING domain
within their N-terminal RBCC motif is in most cases functional and used by the individual TRIM
proteins to polyubiquitinate target proteins followed by their degradation in 26S proteasomes. About
half of the members of this large protein family are connected with the development, progression or
metastasis of tumors (Figure 1). Many TRIM proteins are overexpressed or down regulated in the
diferent cancers (Figure 1) and some TRIM proteins have even been postulated to be prognostic
factors or potential therapeutic targets. Despite their widespread association with carcinogenesis,
the individual TRIM proteins may difer in the way they exert their efects in cancer. Several TRIM
proteins are part of chromosomal rearrangements. For example, TRIM19, also known as PML
(promyelocytic leukemia), is fused to the retinoic acid receptor-α in acute promyelocytic leukemia
[1] and TRIM24, TRIM27 or TRIM33 are fused to the RET (rearranged during transfection)
protein in papillary thyroid carcinoma [2,3]. TRIM24 is also found fused to the fbroblast growth
factor receptor 1 in the myeloproliferative syndrome or to the B-Raf protein in hepatocarcinomas
[3]. Other chromosomal rearrangements that involve TRIM proteins are the fusion of TRIM4 to
the MET kinase in melanomas and the fusion of TRIM46 to MUC1 (Mucin glycoprotein 1) and
KRTCAP2 (keratinocyte associated protein 2) in high-grade serous ovarian cancer [4,5].
Other TRIM proteins are involved in carcinogenesis by controlling the abundance and/or the
activity of p53, an important tumor suppressor protein [6]. Many of these TRIM proteins reduce
p53 level and activity. Among them are TRIM24, TRIM32, TRIM39 and TRIM59, all of which
reduce p53 activity by targeting the tumor suppressor protein for proteasomal degradation. TRIM
proteins such as TRIM21, TRIM25, TRIM28, TRIM29 also reduce p53 activity but through diferent
mechanisms. TRIM21, for instance, indirectly regulates the rapid degradation of p53 by controlling
the subcellular localization of the guanine monophosphate synthase (GMPS) and the herpes virus-
associated ubiquitin protease (HAUSP). Normally, TRIM21 sequesters GMPS in the cytoplasm
through monoubiquitination while HAUSP is localized in the nucleus where it causes degradation
of p53 by stabilizing Mdm2, the major ubiquitin ligase for p53. Upon genotoxic stress, TRIM21 is
released from GMPS allowing the latter to enter the nucleus to displace Mdm2 from its interaction
with p53 and HAUSP, leading to p53 stabilization [7,6]. In another case, TRIM25 suppresses p53
activity by down regulating the activity of p300, a histone acetyl transferase that acetylates p53, a
post-translational modifcation that is mandatory for transcriptional activation of several p53 target
genes [8,9]. TRIM28, on the other hand, interacts with Mdm2 and promotes Mdm2-mediated
ubiquitination and degradation of p53 [10]. In addition, TRIM28 enhances the association of
HDAC1 (histone deacetylase 1) and p53 to promote p53 deacetylation [6]. A diferent mechanism
is used by TRIM29 that sequesters p53 in the cytoplasm keeping it away from the promoters of its
target genes [6]. TRIM29 further promotes the proteasomal degradation of the acetyl transferase
Tip60 leading to a decreased acetylation of p53 at lysine 120, a post-translational modifcation that
is required for the transcriptional activation of the p53 target genes bax and puma and subsequent
initiation of apoptosis [9,11]. TRIM66, one of the few TRIM protein with a non-functional RING
Seham Elabd
1,2
, Andrew C. B. Cato
1
and Christine Blattner
1
*
1
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Germany
2
Department of Human Physiology, Medical Research Institute, Alexandria University, Egypt