Research J. Pharm. and Tech. 13(11): November 2020 5455 ISSN 0974-3618 (Print) www.rjptonline.org 0974-360X (Online) RESEARCH ARTICLE Effect of Extract Marine Sponge Xestospongia sp. and Aaptos sp. on the Plasma IL-1β Level in Inflammatory Model Rats: Time Dependent I Putu Sudayasa 1 , Adryan Fristiohady 2 , Wahyuni 2 , Baru Sadarun 3 , Mentarry Bafadal 2 , La Ode Muhammad Julian Purnama 2 , Nur Syifa Rahmatika 1 , Wa Ode Indri Lestari Kalimin 1 , I. Sahidin 2* 1 Faculty of Medicine, Universitas Halu Oleo, Kendari 93232 Southeast Sulawesi, Indonesia. 2 Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232 Southeast Sulawesi, Indonesia. 3 Faculty of Fisheries and Marine Sciences, Universitas Halu Oleo, Kendari 93232 Southeast Sulawesi, Indonesia *Corresponding Author E-mail: sahidin02@uho.ac.id ABSTRACT: IL-1β is one of the specific biological agents responsible for an inflammatory process. This study aims to investigate the effect of both extract Xestospongia sp. and Aaptos sp. in decreasing plasma IL-1β level in the given time. Xestospongia sp. and Aaptos sp. were extracted by 96% ethanol. Each extract was then suspended into distilled water resulting in various concentrations (50; 100; and 200ppm). Rats were induced by 1% carrageenan intraplantar then were divided into groups (n = 5, each group). After being left for 1 hour, animals treated orally by Xestospongia sp. 50 ppm, 100ppm, and 200ppm; Aaptos sp. 50ppm; 100ppm; and 200ppm), vehicle 0.5% NaCMC (as negative control), and diclofenac sodium 3598 ppm (as positive control). Blood collection was conducted 2 and 3 hours post-treated, then were analyzed by using the ELISA kit. The result showed that Xestospongia sp. 100 and 200ppm and Aaptos sp. were decreasing plasma IL-1β levels at 2 and 3- hour of measurement (p<0.05). In conclusion, both Xestopongia sp. and Aaptos sp. have biological activity as anti-inflammatory agents by decreasing plasma IL-1β levels. KEYWORDS: Xestopongia sp., Aaptos sp., Anti-inflammatory, Plasma IL-1β., Marine Sponge. INTRODUCTION: Inflammatory is the response of the host to eliminate antigen by the host. The inflammatory process involves various process, one of them is involving inflammatory mediators. IL-1β is one of the inflammatory mediators responsible for the inflammatory process. Inflammatory is characterized by tumor, calor, dolor, rubor, and functiolesia, which are disturbing activities hence anti- inflammatory drugs needed. However, the consumption of anti-inflammatory drugs for the long-term has a great side effect such as increasing the risk of infection, hypertension, glaucoma, etc 1,2 . IL-1β is one of the specific biological agents responsible for the inflammatory process. IL-1β is release from the body and rise within 15 minutes, and reaches the peak at 4 hours post-injury 3 . Received on 09.11.2019 Modified on 03.02.2020 Accepted on 25.03.2020 © RJPT All right reserved Research J. Pharm. and Tech. 2020; 13(11):5455-5458. DOI: 10.5958/0974-360X.2020.00952.X Discovering novel drugs from natural products as an alternative to minimize the great side effect of anti- inflammatory drugs is an option. Terrestrial and marine biodiversity of Indonesia as natural resources is abundant. These natural resources can be utilized in many aspects which is one of the aspects is as traditional medicine. Indonesian people themselves have been well- known to utilize natural resources as traditional medicine. According to Riskerdas 2018, 31.4% of people utilize traditional medicine as self-medication efforts and adjuvant medical treatment 4 . The utilization of terrestrial and marine biodiversity as traditional medication can lead to the development of novel drugs. Marine sponges have many novel natural products with various chemical structure and biological activities hence marine sponges such as Aaptos sp. can be developed and researched further for its potencies. Based on the previous study, Xestospongia sp. and Aaptos sp have been reported act as an antibacterial, antifungal, anticancer, antidepressant, anti-HIV-1, and