S1   For                       !"# $%&’ Jianxian Sun a , Song Tang b , Hui Peng* ,a , David M.V. Saunders a , Jon A. Doering a , Markus Hecker a,b , Paul D. Jones a,b , John P. Giesy a,c,d,e,f , and Steve Wiseman* ,a a Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada b School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C8, Canada c Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada d Zoology Department, Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan 48824, USA e School of Biological Sciences, University of Hong Kong, Hong Kong Special Administrative Region 999077, People’s Republic of China f State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, People’s Republic of China *& ( Hui Peng, huisci@gmail.com, Tel.: +1 306 966 8733; Fax: +1 306 966 4796. Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada; Steve Wiseman: steve.wiseman@uleth.ca, Tel: (403) 329D2320, Fax: (403) 329D2082. Department of Biology, University of Lethbridge, Lethbridge, Alberta T1K 3M4, Canada. Words 1,798 Tables 1 Figure 3 This supporting information provides text of details, tables and figures addressing (1) RNA sequencing; (2) Protein preparation and identification; (3) Identification of proteins by mass spectrometry; (4) Analysis of TBCO; (5) Visual performance assessment; (6) Cardiac function assessment; (7) Concentrations of TBCO in exposure media prior to mediaDchange; (8) Structures of HBCD and TBCO; (9) Heatmap of significantly altered transcripts and proteins in embryos exposed to control and 100 Gg/L TBCO; (10) Biplots illustrating diatanceDbased redundancy analysis (dbRDA) of transcriptome and proteome, and regression between transcripts and proteins from control samples.