1 In Vitro Investigation of Therapeutic and Anti-Coagulant Properties of Allium Sativum L. On Human Plasma Sadaf Sarfraz, Muhammad Ismaeel, Shomaila Sikandar Department of Chemistry, Lahore Garrison University Corresponding author’s email: ismailmeo@ymail.com This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: https://doi.org/10.32350/BSR.0401.06 Received: November 12, 2021; Revised: March 15 2022; Accepted: March 31, 2022 ABSTRACT Cardio vascular illnesses are increasing worldwide and among these illnesses Thromboembolic disorders are very prevalent. The anticoagulant properties of many herbs have been reported. Garlic is a common herbal supplement. The current study was conducted to compare the in vitro anti-coagulant activity of different solvent-extracted fractions on human blood samples. For this purpose, rectified spirit and reverse osmosis (RO water), were used as solvents. Multiple extracting approaches were applied to prepare different extract fractions of Allium sativum, both in ethanolic and aqueous extracts by maceration, decoction, and soxhlet extraction methods. The concentration of each extract fraction was subjected to a primarily anti-coagulant screening method in human blood sample in-vitro by calculating their prothrombin time of coagulation. . The anti-coagulant activity of the extracts was determined by measuring the changes in prothrombin time with a null hypothesis value of p< 0.05. The results indicated that all garlic extract fractions had significant Copyright © 2021 The Authors. Production and hosting by Department of Life Sciences, University of Management and Technology is licensed under a Creative Commons Attribution 4.0 International License