Natural Resources, 2019, 10, 299-304 http://www.scirp.org/journal/nr ISSN Online: 2158-7086 ISSN Print: 2158-706X DOI: 10.4236/nr.2019.108019 Aug. 22, 2019 299 Natural Resources New Method for Isolation of Naringin Compound from Citrus maxima Aliefman Hakim, Jamaluddin, I. Nyoman Loka, Sukib, Ni Wayan Wida Sasmining Prastiwi University of Mataram, Mataram, Indonesia Abstract This research aims to know the proper isolation method to isolate naringin compound from Citrus maxima for natural product chemistry laboratory. This natural product chemistry laboratory provides students with the essen- tial skills and knowledge required to perform the extraction, isolation, and structural identification. The isolation began with sample preparation, extrac- tion with methanol, partition with n-hexane, purification by crystallization using isopropanol, by analyzing the purity with Thin Layer Chromatography (TLC) tested, and the presence of functional groups with Fourier Transform Infrared (FT-IR) spectrophotometer test. The result of FT-IR spectrophoto- meter analysis contains hydroxy group (-OH), aromatic cyclic groups (C=C), CH 2 , CH 3 , and ether groups. Keywords Isolation, Naringin, Citrus maxima 1. Introduction Indonesia is a country with abundant biodiversity. The diversity of animals and plants is diverse. The diversity of plants is a source of secondary metabolite compounds that have been known to have many benefits [1]. Indonesian people also have long known that some plants are processed into herbs. Secondary me- tabolite compounds contained in plants can be obtained by the implementation of isolation method [2] [3]. The biodiversity is a source of chemical compounds. The primary metabolite compounds such as proteins, carbohydrates, fats used by plants for their growth, and secondary metabolites such as terpenoids, stero- ids, polyphenols, flavonoids and alkaloids serve as a protector of plants [4]. Sec- ondary metabolites are produced from various organisms such as plants, bacte- ria, and fungi. Chemical compounds as a result of secondary metabolites in var- How to cite this paper: Hakim, A., Jama- luddin, Loka, I.N., Sukib and Prastiwi, N.W.W.S. (2019) New Method for Isolation of Naringin Compound from Citrus max- ima. Natural Resources, 10, 299-304. https://doi.org/10.4236/nr.2019.108019 Received: July 23, 2019 Accepted: August 19, 2019 Published: August 22, 2019 Copyright © 2019 by author(s) and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access