International Journal of Research in Medical Sciences | April-June 2014 | Vol 2 | Issue 2 Page 457 International Journal of Research in Medical Sciences Kannan I et al. Int J Res Med Sci. 2014 May;2(2):457-461 www.msjonline.org pISSN 2320-6071 | eISSN 2320-6012 Research Article Isolation and antibiotic susceptibility of bacteria from foot infections in the patients with diabetes mellitus type I and type II in the district of Kancheepuram, Tamil Nadu, India Kannan Iyanar*, Premavathy R. K., Sambandam Cecilia, Jayalakshmi M., Sruthi Priyadarsini S., Shantha S. INTRODUCTION Diabetes mellitus is a worldwide disorder and affects many people. Type 2 diabetes is the most common form of diabetes in developing countries like India, Africa and China. At present 31.7 million people are diabetic in India. Hence, it has been labelled as “The diabetic capital of the world”. 1 Diabetes is considered to be a serious disorder not just for its predominance but for its complications. One such important complication is the ABSTRACT Background: Diabetic foot infections are important cause of morbidity and mortality among persons with diabetes mellitus. The reported prevalence rates in India range from 0.9–8.3%. Diabetes foot lesions are the leading cause of non-traumatic amputations worldwide. A study has been conducted to isolate and find the antibiotic susceptibility pattern of the bacteria from diabetic foot infections from the patients of Kancheepuram district, Tamil Nadu, India. Methods: Sixty patients previously diagnosed or newly diagnosed as diabetic, presented with lower extremity infection attending Tagore medical college and hospital and its peripheral centres were selected for the study. Various specimens (pus, wound exudates, or tissues biopsy) for microbiological studies were obtained from the infected region. The specimens were cultured on blood agar and MacConkey agar for aerobic / facultative anaerobic organisms and on Neomycin Blood Agar for anaerobic organism. The plates were then incubated at 37°C. For anaerobic culture the plates were incubated in the McIntosh anaerobic jar. Isolates obtained are identified by standard laboratory techniques. Results: The result showed that Pseudomonas aeruginosa (48.3%) is the predominant bacterium followed by Staphylococcus aureus (38%) and other bacteria. The anaerobic bacteria are also isolated from the diabetic foot ulcers. The Peptostreptococcus species (26.7%) are the predominant bacteria followed by other bacteria. Further the results showed that 22 patients (37%) showed the multi-bacterial infection and remaining 38 patients (63%) showed mono bacterial infection. The drugs like amikacin, cefepine, ciprofloxacin, cotrimoxazole and roxythromycin are sensitive to many gram positive bacterial isolates. Conclusion: The present study has given the data of various bacteria encountered in the diabetic foot ulcer in the district of Kancheepuram, Tamil Nadu, India and its antibiotic sensitivity pattern. The results clearly reveal that there is no definite aetiology in diabetic foot infections. Many patients presented the infection with the involvement of many bacteria. Further it is evident that many bacteria are multi drug resistant and thus complicating the management of diabetic foot infections. Keywords: Diabetic foot infection, Bacterial isolates, Antibiotic sensitivity pattern, Kancheepuram district Department of Microbiology, Tagore Medical College and Hospital, Chennai - 600127, Tamil Nadu, India Received: 03 January 2014 Accepted: 02 February 2014 *Correspondence: Dr. Kannan Iyanar, E-mail: kannan_iyan@hotmail.com © 2014 Kannan I et al. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. DOI: 10.5455/2320-6012.ijrms20140515