Citaton: Busman H, Lestari RP, Rosa E, Arifyanto A. Kersen (Muntngia calabura L.) Ethanol Extract Repairs Pancreatc Cell Damage, Total Coliforms, and Lactc Acid Bacteria in Hyperglycemic Mice. J Pure Appl Microbiol. 2022;16(3):1943-1952. doi: 10.22207/JPAM.16.3.41 © The Author(s) 2022. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Busman et al. | Artcle 7883 J Pure Appl Microbiol. 2022;16(3):1943-1952. doi: 10.22207/JPAM.16.3.41 Received: 07 June 2022 | Accepted: 16 July 2022 Published Online: 12 August 2022 ReSeARCH ARtiCle OPeN ACCeSS www.microbiologyjournal.org 1943 Journal of Pure and Applied Microbiology P-ISSN: 0973-7510; E-ISSN: 2581-690X *Correspondence: achmad.arifyanto@fmipa.unila.ac.id Kersen (Muntngia calabura l.) ethanol extract Repairs Pancreatc Cell Damage, total Coliforms, and lactc Acid Bacteria in Hyperglycemic Mice Hendri Busman, Reza Pina lestari, emants Rosa and Achmad Arifyanto* Department of Biology, Faculty of Mathematcs and Natural Sciences, University of Lampung, Jl. Sumantri Brojonegoro No. 1. Bandar Lampung, Lampung, Indonesia - 35145. Abstract Hyperglycemia is a conditon in which the blood glucose levels exceed normal limits. ethanol extract of Jamaican cherry leaves (Muntngia calabura l.) contains actve components that have the potental to lower blood sugar levels and heal pancreatc cell damage in rats. Gut microbiota imbalance can cause immune system abnormalites, illnesses, and metabolic disorders, including diabetes. the aim of the present study was to determine whether an ethanol extract of Jamaican cherry leaves can repair pancreatc cell damage, as well as infuence the total coliforms and lactc acid bacteria in hyperglycemic mice. in this study, 25 mice were randomly placed into fve groups, which were then provided with food and drinking water. Alloxan in a dose of 160 mg/kg bw was administered to the positve control group and three treatment groups. each of the three groups was then treated with the ethanol extract of Jamaican cherry leaves at doses of 300, 400, or 500 mg/kg bw for 14 days. the groups that received the extract were able to repair the pancreatc damage considerably by increasing the number of normal pancreatc cells. this was supported by histological observatons. the total abundance of lactc acid bacteria in hyperglycemic mice was 1.08 ± 45 x 10 10 CFU/g, which was substantally lower than that in mice treated with the extract. the overall coliform abundance in hyperglycemic mice was 4.37 ± 41 x 10 10 CFU/g, which was higher than that in mice supplemented with the extract. therefore, the administraton of Jamaican cherry leaf ethanol extract on a regular basis can increase the abundance of cecum microbiota in hyperglycemic mice. Furthermore, this extract can be used as a natural alternatve treatment for recovering pancreatc cell damage. Keywords: Alloxan, Gut Microbiota, Hyperglycemia, Muntngia Calabura, Pancreatc Cells