* To whom all correspondence should be addressed. E-mail: yashab.kumar@shiats.edu.in JOURNAL OF PURE AND APPLIED MICROBIOLOGY, March 2017. Vol. 11(1), p. 499-504 Comparative Study Between Different Types of Media used for the Isolation of Uropathogens with Special Reference to E.coli Yashab Kumar, Lata Kunj and Pramod W. Ramteke Sam Higginbottom Institute of Agriculture, Technology and Sciences, Deemed to be University, Allahabad - 211007, India. http://dx.doi.org/10.22207/JPAM.11.1.65 (Received: 12 September 2016; accepted: 03 December 2016) Escherichia coli is the greatest cause of primary urinary tract infections. The present study aimed Urinary tract infection in female with special reference Escherichia coli and its antibiotic susceptibility pattern. A total of 100 urine samples were collected from different hospitals of Allahabad. The samples were cultured on Cystein lysine electrolytes deficiency media, MacConkey’s agar, Nutrient agar and Hicrome UTI agar media. Based on the results it was found that different media had different properties and Hicrome UTI agar was the best and convenient media for the isolation of Escherichia coli and other uropathogens. Further it was also observed that Escherichia coli was the most common organism for UTI in female. Keywords: Urinary tract infection, Female, Escherichia coli, Nutrient agar, MacConkey’s agar, Cystein lysine electrolytes deficiency, Hicrome UTI agar. Urinary tract infections are among the most common bacterial infections that lead patients to seek medical attention and it has large socio- economic impacts. It is also one of the most common infectious diseases diagnosed in outpatients as well as in hospitalized patients and can lead to significant mortality. (Adedeji and Abdulkadir., 2009). Urinary tract infection (UTI) is an infection of one or more components of the urinary tract. Many infection resolve spontaneously but other can progress to destroy the kidney. Urinary tract is made up of two sections: the lower urinary tract and the upper urinary tract. Lower urinary tract contains the bladder and urethra. Upper urinary tract contains two kidneys and the tube that connects them, called the ureters. Urine does not normally contain microorganisms. Most urinary tract infections are due to bacteria that enter the opening of the urethra. Bacteria stick to the walls of the urethra, multiplying and moving up the urethra to the bladder. Most UTIs remain in the lower urinary tract where they cause annoying symptoms, such as a burning sensation during urination. These infections are easily treated in most cases. Subjects were considered to have a culture-confirmed urinary tract infection if they had dysuria, frequency, or urgency (or all three) and e”10 2 colony-forming units of an uropathogen per milliliter of midstream urine (Hooton et al., 1996). Escherichia coli has been documented as the most important pathogen associated with urinary tract infections. The organisms isolated were Escherichia coli , Staphylococcus saprophyticus , Klebsiella aerogenes , Staphylococcus aureus , Pseudomonas aeruginosa, Streptococcus faecalis and Proteus mirabilis (Adedeji and Abdulkadir., 2009).UTI’s more commonly progress to bladder infections in