P.1.g. Basic and clinical neuroscience − Neuropharmacology S243 but overlapping, neural circuitries. Additionally, we investigated the interactive role of NMDA receptors and mGluR5 in modulat- ing 5-CSRTT performance. Following behavioural training, both unscreened rats (5-CSRTT rats, n = 11; DDT rats, n = 20) and rats selected for extreme high (HI; n = 12) and low (LI; n = 11) levels of impulsivity were tested in the 5-CSRTT and DDT fol- lowing systemic ADX47273 administration (a functionally charac- terised positive allosteric modulator of mGluR5; p.o, 2 ml/kg, 40– 100 mg/kg). Furthermore, the effect of ADX47273 (p.o, 2 ml/kg, 80–100 mg/kg) in a pharmacologically-evoked model of impul- sivity, by NMDA receptor antagonism (MK801; s.c, 1ml/kg, 0.03mg/kg), was also investigated in mid-impulsive (MI) rats (n=11−12 per group) in the 5-CSRTT. To test for non-specific motoric effects of ADX47273, spontaneous locomotor activity was also assessed in a sub-group of rats (n = 6−8 per group) following ADX47273 pre-treatment (p.o, 2 ml/kg, 60–100 mg/kg). ADX47273 selectively decreased premature responding in the 5-CSRTT, both in unscreened and HI and LI rats, at doses that did not alter spontaneous locomotor activity (unscreened rats, p < 0.05; HI and LI rats; p < 0.01). MK801 robustly increased pre- mature responding and additionally impaired attentional accuracy in MI rats (p < 0.001 and p < 0.01 respectively). These deficits were dose-dependently rescued by ADX47273 pre-treatment. In stark contrast, positive allosteric modulation of mGluR5 had no effect on DDT performance. These findings demonstrate that motor and choice impulsivity, as assessed by the 5-CSRTT and DDT respectively, are dissociable at the level of mGluR5. We further show that positive allosteric modulation of mGluR5 may be therapeutically valuable in selectively targeting specific aspects of maladaptive impulsivity and executive dysfunction. P.1.g.010 Use of chronically administered adipokinetic hormone in depression, anxiety and pain O. Mutlu 1° , G. Ulak 1 , E. Gumuslu 2 , I.K. Celikyurt 1 , F. Akar 1 , F. Erden 1 , P. Tanyeri 3 1 Kocaeli University, Medical Pharmacology − Psychopharmacology lab. − Medical Faculty, Kocaeli, Turkey; 2 Kocaeli University, Medical Genetics − Medical Faculty, Kocaeli, Turkey; 3 Sakarya University, Pharmacology − Medical Faculty, Sakarya, Turkey A great variety of processes in insects is known to be influenced or regulated by neuropeptides. These peptide hormones are prod- ucts of neurosecretory neurons located in the corpora cardiaca, neuroendocrine glands attached to the brain. One of the major neuropeptide groups is the adipokinetic (AKH) and hypertre- halosaemic (HrTH) peptides. They control fat, carbohydrate and protein metabolism. The aim of this study was to investigate the effects of Anax imperator (Ani-AKH) (1 and 2 mg/kg) (n = 9−10), Libellula auripennis (Lia-AKH) (1 and 2 mg/kg) (n = 10−12) and Phormia-Terra (Pht-HrTH) (1 and 2 mg/kg) (n = 10−12) or vehicle (saline with %1 DMSO) (n = 10) administered for 14 days on depression, anxiety, pain and locomotion in the forced swimming (FST), elevated plus maze (EPM), hot plate and locomotor activity tests. In the FST test, the duration of immobility was recorded during the last 4 min of the 6-min testing period. In the plus maze test, the open-arm activity was evaluated. In the hot plate test, the latency of licking of the hind paws or jumping was recorded as an index of pain reaction. In the FST test, Ani-AKH (1 and 2 mg/kg) (p < 0.001), Lia-AKH (1 and 2 mg/kg) (p < 0.001) and Pht-HrTH (1 and 2 mg/kg) (p < 0.001) significantly diminished immobility time compared to control group. In the EPM test, Ani- AKH (1 and 2 mg/kg) didn’t alter %time spent in the open arms and %open arm/total arm entries. Lia-AKH (2 mg/kg) (p < 0.05) and Pht-HrTH (1 and 2 mg/kg) (p < 0.05) significantly increased %time spent in the open arms while Lia-AKH (2 mg/kg) (p < 0.01) and Pht-HrTH (1 and 2 mg/kg) (p < 0.001) significantly increased %open arm/total arm entries. In the hot plate test, Ani-AKH (1 and 2 mg/kg) (p < 0.05) significantly increased latency for the first time licking their hindpaws compared to control. Pht- HrTH (2 mg/kg) (p < 0.05) and Lia-AKH increased latency for the first time licking their hindpaws compared to control although the effect of Lia-AKH was not significant. In the locomotor activity test, Ani-AKH (2 mg/kg) (p < 0.01) increased the total distance moved compared to control and increased the speed of the animals. Lia-AKH (1 and 2 mg/kg) (p < 0.05) and Pht-HrTH increased the total distance moved and increased the speed of the animals although the effect of Pht-HrTH was not signif- icant. Ani-AKH (2 mg/kg), Lia-AKH (2 mg/kg) and Pht-HrTH (2 mg/kg) upregulated brain-derived neurotrophic factor (BDNF) and cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) messenger ribonucleic acid (mRNA) levels in the hippocampus in the quantitative real-time polymerase chain reaction (RT-PCR) test. Our study showed that Lia-AKH and Pht-HrTH had anxiolytic effects when given chronically in the elevated plus maze test. Ani-AKH, Lia-AKH, Pht-HrTH had antidepressant effects in the forced swimming test. Ani-AKH and Pht-HrTH had antinociceptive effects in the hot plate test. Ani-AKH and Lia-AKH had locomotion enhancing effects in the locomotor activity test. P.1.g.011 Antinociceptive effects of ketamine and alpha-lipoic acid in rats with neuropathic pain N. Doncheva 1° , L. Vasileva 1 , K. Saracheva 1 , D. Getova 1 1 Medical University, Pharmacology, Plovdiv, Bulgaria Purpose: Neuropathic pain is defined by the IASP as “pain initiated or caused by a primary lesion or dysfunction of the nervous system” [1]. Diclofenac, COX inhibitor, possesses anal- gesic effect by inhibiting COX-2 enzyme and thus the synthe- sis of prostaglandins, leukotrienes and thromboxanes. Activation of NMDA receptor is usually involved in the development of spinal sensitization and NMDA receptor antagonists are used in the management of neuropathic pain. Ketamine is a non- competitive blocker for the glutamate NMDA receptors with analgesic, dissociative and neuroprotective effects. Alpha-lipoic acid is a lipophilic antioxidant, which used to treat diseases in which oxidative stress plays a major role. Methods: Forty rats were randomized into five groups. Group I (sham) and Group II (control) received saline (0.1 ml, in- traperitoneally), Group III − Diclofenac 25 mg/kg, Group IV − Ketamine 50 mg/kg and Group V − Alpha-lipoic acid 60 mg/kg intraperitoneally. Peripheral neuropathic pain was induced in rats by chronic constriction injury under common anesthesia. Two loose ligatures were placed around the sciatic nerve to induce neuropathic pain. Drug application started on the next day af- ter operation. Seven days after ligature of the sciatic nerve, the antinociceptive effect was evaluated by using hot-plate and Randall-Sellito tests [2]. The hot-plate test evaluates the reaction time of rats in seconds with are dropped on a heated surface. The