ORIGINAL RESEARCH
Journal of Dentomaxillofacial Science (J Dentomaxillofac Sci ) December 2020, Volume 5, Number 3: 158-161
P-ISSN.2503-0817, E-ISSN.2503-0825
158 © 2020 JDMFS. Published by Faculty of Dentistry, Hasanuddin University. All rights reserved. http://jdmfs.org
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Ab stract
Objective: To compare the osteoblast cell formation process after
installation implant with and without added of platelet-rich plasma (PRP).
Methods: Twenty-four male rabbit were selected by purposive
sampling, divide into two groups. Group 1 implant with added PRP
were installation in the distal part of the thigh bone 12 male rabbits,
Group 2 implants without the addition of PRP mounted on the distal
part of the thigh bone 12 male rabbits. All rabbits in euthanasia after 0,
7, 14, and 28 days were then analyzed histologically to determine the
formation of osteoblast cells.
Results: There is increased formation of osteoblast cell formation in
implant installation with the addition of PRP when compared with
implants without PRP as time increases.
Conclusion: Administration of PRP in implants can speed up and
increase osteoblast cell formation.
Keywords: Bone remodeling, Implant, Platelet- rich plasma
Cite this Article: Machmud E, Thalib B, Dharmautama M, Mude AH, Dammar I, Ikbal M. 2020. Platelet-rich plasma improves initial bone remodeling.
Journal of Dentomaxillofacial Science 5(3): 158-161. DOI: 10.15562/jdmfs.v5i3.688
Platelet-rich plasma improves initial bone
remodeling
Edy Machmud,
*
Bahruddin Thalib, Mochammad Dharmautama,
Acing H. Mude, Irfan Dammar, Muhammad Ikbal
Introduction
Correction of bone defects in the installation
of dental implants can be accelerated by adding
platelet-rich plasma (PRP) material that can initi-
ate wound healing by releasing locally employed
growth factors.
1-3
Platelet-rich plasma is a protein
taken autologous, using regeneration of the
body itself, so it has the advantage that; PRP
can prevent disease transfer and immunologic
reactions, PRP is easy to obtain because it comes
from self-administered animal blood, time is
short, easy and safe to apply, PRP has high fbrin
content, PRP accelerates epithelial, endothelial
and epidermal regeneration which can promote
tissue healing through angiogenesis and collagen
synthesis, PRP can accelerate bone formation and
maturation.
4
Bone formation begins with the release of
platelet derive growth factor (PDGF), TGF and
IGF. Vascular endothelial growth factor increases
endothelial growth proliferation. Platelet derived
growth factor will bind to endothelial cells to initi-
ate wound healing through collagenase activity.
Transforming growth factor-beta (TGF-B) will
bind to osteoblasts and stem cells to stimulate oste-
oid production resulting in bone resorption and
woven bone formation.
5
PRP is a platelet in gel form extracted through
centrifugation of bovine thrombin mixture,
10% calcium chloride and venous blood. PRP
also contains signifcant growth factor concen-
trations that will increase bone formation and
mineralization, induce stem cells to diferentiate
into osteoblasts, minimize bone resorption, increase
angiogenesis and produce collagen through
fbroblast activation.
6,7
Te purpose of this study was to analyze the
efect of the addition of PRP on the installation
of dental implants to the initial bone remodeling.
Tis research is almost similar to the research done
by Brunamelia de Oliviera on the evaluation of
biomaterials with and without platelet-rich plasma:
a histometric study using beagle dogs, but diferent
in terms of methods, and research samples. In this
study only use the time from the frst day to the
fourteenth day because only want to see the initial
bone formation.
Material and Methods
Twenty four New Zealand rabbits, aged 4-8 months,
weight 1500-2000 grams maintained for seven days
in clean, air-free cage, fed and drank three times
a day. Divided into two groups, the frst group
contained twelve rabbits placed implanted with
PRP on her thighbone, the second group containing
12 rabbits placed an implant without PRP on
her thighbone. Furthermore each 3 rabbits will be
sacrifced afer 1, 3, 7, and 14 days afer implant
installation both in frst and second group. Intake of
bone tissue around the implantation area, making
histologic preparations to calculate the number of
osteoblast cells using an Optilab microscope Raster
V3 image with magnifcation 10, 40 and 100.
Department of Prosthodontics,
Faculty of Dentistry Hasanuddin
University, Makassar, Indonesia
*
Correspondence to: Edy Machmud,
Department of Prosthodontics,
Faculty of Dentistry Hasanuddin
University, Makassar, Indonesia
machmudedy@gmail.com
Received: 11 April 2018
Revised: 20 August 2019
Accepted: 15 December 2019
Available Online: 1 December 2020