Clinical and translational S53 differences between neutral and sad mood with baseline cognitive reactivity and rumination scores. Results: After sad vs. neutral-mood-induction, remitted MDD patients showed attenuated connectivity between the posterior DMN and the hippocampus; posterior DMN- hippocampal connectivity was increased in controls, but not in remitted-MDD patients. Increased hippocampal connectivity was associated with lower levels of cognitive reactivity (r = −21, p = 0.046) and rumination (r = −27, p = 0.017). No effects of sad versus neutral mood-induction were observed in the anterior-DMN, salience- or central executive networks. Conclusion: We showed that in un-medicated MDD- patients in remission, there was attenuated posterior- DMN-connectivity with the hippocampus after sad autobiographical recall. This attenuated connectivity was associated with postulated risk-factors for recurrence; rumination and CR. We interpret our findings in the perspective of over-general autobiographical memory processing in remitted-MDD, where remitted-MDD remember less detailed, more generic memories, with rumination as an underlying factor4. This study adds to evidence pointing to the DMN as an important neural network that is persistently dysfunctional during remission in the presence of stressful triggers. Future studies should assess whether attenuated hippocampus connectivity after sad auto-biographical recall is predictive of recurrence. Reference(s) [1] Watkins, ER., 2008. Constructive and Unconstructive Repetitive Thought. Psychological Bulletin, 134(2), 163–206. [2] Figueroa, C.A., Ruh´ e, H.G., Koeter M.W., Spin- hoven, P., Van der Does, W., 2015. Cognitive Reactivity Versus Dysfunctional Cognitions and the Prediction of Relapse in Recurrent Major Depressive Disorder. J Clin Psychiatry http://dx.doi.org/10.4088/ JCP.14m09268. [3] Marchetti, I., Koster, E.H., Sonuga-Barke, E.J., De Raedt, R., 2012. The default mode network and recurrent depression: a neurobiological model of cognitive risk factors. Neuropsychol Rev, 22(3), 229– 251. [4] Raes, F., Schoofs, H., Griffith, J.W., & Hermans, D., 2012. Rumination relates to reduced autobiographical memory specificity in formerly depressed patients following a self-discrepancy challenge: the case of autobiographical memory specificity reactivity. J Behav Ther Exp Psychiatry, 43(4), 1002–1007. P.3.009 Quitting cannabis decreases but does not eliminate psychosis risk: evidence from a 7 years large population-based cohort U. Kirli 1 ° , T. Binbay 2 , H. Elbi 1 , B. Kayahan 1 , D. Gokcelli 1 , K. Alptekin 2 , J. Van Os 3 . 1 Ege University School of Medicine, Department of Psychiatry, Izmir, Turkey; 2 Dokuz Eylul University School of Medicine, Department of Psychiatry, Izmir, Turkey; 3 Maastricht University Medical Centre, School of Mental Health and Neuroscience, Department of Psychiatry and Neuropsychology, Maastricht, The Netherlands Introduction: Cannabis is known to be a risk factor for both psychotic experiences and schizophrenia [1]. It is clear that some of the similarities in the neurobiology of cannabis and schizophrenia may indicate a mechanism for the development of psychosis [2]. However, the association between cannabis use and other forms of clinical psychosis has been less studied. Purpose: Investigating differential impact of continuum and frequency of cannabis use across the onset of any DSM disorder with clinical features in a 7 years follow-up of an epidemiological sample. Method: Addresses representing third biggest city center of Turkey (Izmir) were contacted in multistage clustered area probability sampling frame covering 11 districts and 302 neighbourhoods. The sample was screened with the Composite International Diagnostic Interview 2.1 for psychotic experiences and cannabis use; demographic variables were recorded and SCID-I was conducted to symptomatic participants at baseline (T1) and 7 years after (T2) (n: 2142). Associations between cannabis use variables and newly onset clinical psychosis were tested using logistic regression models controlling for age, gender, education status and health insurance. Results: Sociodemographic risk factors were associated with newly onset clinical psychosis: Being at the age of 21−35 (OR: 4.8, 95% CI:1.4–14.6; p < 0.05), divorced (OR:13.1 95% CI: 4.7–36.6, p < 0.05), unem- ployed (OR:3.5, 95% CI: 1.1–10.9 p < 0.05), without health insurance (OR: 4.5, 95% CI:1.8–11.5 p < 0.05), in lower socio-economic status (OR: 5.7, 95% CI:1.3–24.3 p < 0.05). Continuum of cannabis use was associated with 33 fold increased risk: usage before seven years and for the last seven years (OR: 33.2, 95% CI: 6.1–181.6, p < 0.05). Quitting cannabis use decreased the risk but significant proportion of risk remained: usage before seven years but no use for the last seven years (OR: 20.8, 95% CI: 2.2– 194.2, p < 0.05). Cannabis use starting in last seven years was also associated with newly onset clinical psychosis (OR: 16.6, 95% CI: 5.2–53.0, p < 0.05). Frequency of