365 ZIRAA’AH, Volume 44 Nomor 3, Oktober 2019 Halaman 365-376 p-ISSN 1412-1468 e-ISSN 2355-3545 PENGARUH JUMLAH DAUN DAN KONSENTRASI ROOTONE-F TERHADAP PERTUMBUHAN BIBIT JERUK NIPIS LEMON (Citrus limon L.) ASAL STEK PUCUK The Effect of the Number of Leaves and Concentration of Rootone-F on the Growth of Lemon Lime (Citrus limon L.) Seedling from Shoot Cuttage) Puspita Sari, Yazid Ismi Intara, dan Alvera Prihatini Dewi Nazari Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Mulawarman. Jl. Pasir Balengkong, Kampus Gunung Kelua, Samarinda 75119, Kalimantan Timur, Indonesia. E-Mail: Puspitasari7382@gmail.com Article Submitted : 16-08-2019 Article Accepted : 05-10-2019 ABSTRACT The research was conducted to know the interaction between the number of leaves and concentration of Rootone-F; the effect of the number of leaves; and concentration of Rootone-F on the growth of lemon lime seedling from shoot cuttage singly. The experiment was conducted from November 2017 until Febuary 2018, on Faculty of Agriculture, Mulawarman University. The factorial experiment was arranged in Completely Randomized Design with five replications. The first factor was the number of leaves, consisted of: 5, 7 and 9 leaves, while the second factor was concentration of Rootone-F, consisted of: 0.00; 0.05; 0.10; and 0.15 g mL -1 . Variables which observed consisted of: time of bud emergence, the number of buds, the number of leaves, bud length, bud diameter, the number of roots and root length. Data were analyzed by analysis of variance and continued by Least Significant Difference test at 5% of significant level. The result showed that the interaction between the number of leaves and concentration of Rootone-F has no significant on variables observed, unless on bud diameter. The effect of the number of leaves has significantly different on variables observed, however, it has no significant on the number of buds, the number of leaves and bud length at 12 weeks after planting (WAP). The concentration of Rootone-F effect has significantly different on variables observed, unless on the number of buds and the number of leaves at 6 WAP. The best growth of lemon lime seedling obtained by nine leaves and 0.10 g Rootone-F mL -1 singly. Keywords: lemon lime, number of leaves, Rootone-F, shoot cottage PENDAHULUAN Jeruk nipis lemon merupakan salah satu tanaman hortikultura yang cukup dikenal masyarakat, pada umumnya ditanam di pekarangan sebagai tanaman apotek hidup. Buah jeruk nipis lemon mengandung vitamin, mineral dan serat, sering dipakai sebagai penambah rasa dalam kuliner khas daerah, sehingga mempunyai nilai ekonomi yang cukup tinggi. Prospek pemasaran buah jeruk nipis lemon di Kalimantan Timur sangat cerah, namun pengembangan tanaman ini belum maksimal dan perannya dalam perekonomian daerah masih rendah, hal ini ditunjukkan dengan belum adanya data produksi jeruk nipis lemon. Salah satu hambatan dalam pengembangan tanaman ini adalah ketersediaan bibit atau bahan tanam yang berkualitas. Upaya yang dapat dilakukan untuk mengatasi masalah tersebut adalah melalui penerapan teknologi pembibitan yang cepat dan menjamin keseragaman dan kestabilan hasil untuk memenuhi kebutuhan bibit unggul jeruk di