Conclusions: The study elucidates a significant inverse relation of of physical and mental health and disease activity in patients of RA. QoL is a multi-dimensional concept which defines a person's accomplishment of life's goals, so it may be imperative to sensitize clinicians for periodic assessment of QoL for a holistic disease management in patients of RA. P60. Soluble CD14: a promising marker for assessing disease severity in rheumatoid arthritis Sarit Sekhar Pattnaik a , PrasanaSamal a , Aditya Panda b , Rina Tripathy a , Bidyut Das a ; a Department of Medicine, Unit of Clinical Immunology and Rheumatology, SCB Medical College, Cuttack, India; b Center of Life Sciences, Central Institute of Jharkhand, Ranchi, India Background: Soluble CD14 (sCD14) is an important molecule of innate immunity and has been linked to various autoimmune disorders including rheumatoid arthritis (RA). However, investi- gation on association between sCD14 levels and disease severity is very limited. Methods: 140 consecutive RA patients diagnosed on the basis of ACR classification criteria for RA were enrolled in this study. Disease activity was assessed by DAS28, SDAI and CDAI. Func- tional status was assessed by HAQ score. Age and sex matched healthy controls (n¼80) were also included. Plasma levels of sCD14, TNF-a, IL-6 and IL-1b was quantified by ELISA. Biochem- ical, hematological and serological parameters were evaluated by standard laboratory procedures. Data was analysed for statistical significance by Prism Graph pad software. Results: The profile of the cohort showed there were more females (n¼129) than males (n¼11) with a mean age of 44.7± 10.9 years. Plasma sCD14 levels were significantly high in RA patients compared to healthy controls (P < 0.0001). Further, sCD14 levels positively correlated with disease activity scores [DAS28:P<0.0001, r¼0.41; SDAI:P<0.0001, r¼0.42; CDAI:P<0.0001, r¼0.43; and HAQ score: P¼0.0001, r¼0.31], Furthermore, plasma levels of sCD14 positively correlated with plasma TNF-a (P<0.0001, r¼0.61), IL-6 (P<0.0001, r¼0.42) and IL-1b (P¼0.04, r¼0.17) . Besides plasma sCD14 levels significantly correlated with ESR and CRP (p¼0.04, r¼0.17) indicating its role as an important biomarker in assessing disease severity. Conclusions: Plasma sCD14 is a promising biomarker in the assessment of RA disease and functional severity. P61. Low serum vitamin D (25-OH) levels is associated with severe disease activity and poor functional status in patients of RA Rashmi Ranjan Sahoo a , Sunita Sethy a , Rina Tripathy b , Sarit Sekhar Pattnaik a , Bidyut Das a ; a Department of Medicine, Unit of Clinical Immunology and Rheumatology, India; b Department of Biochemistry, SCB Medical College, Cuttack, India Introduction: Rheumatoid arthritis (RA) is a chronic autoimmune disease of unknown etiology affecting women in the reproductive age group. Vitamin D deficiency has been found to increase the risk of developing RA. Furthermore role of vitamin D deficiency in the pathogenesis of RA and disease activity has been elucidated. There are few reports from Indian population. This study aims to assess levels of vitamin D and correlate its levels with disease activity in patients of RA. Methods: 104 consecutive RA patients fulfilling 2010 ACR-EULAR criteria and 96 age matched healthy controls were enrolled in the study. Disease activity was assessed by DAS28, SDAI and CDAI. Functional assessment was done by HAQ score .Blood was collected for routine hematological, biochemical investigations and serum vitamin D estimation was done by ELISA kit (Euro- immune, Germany).Statistical analysis was done using Graphpad prism. Results: There were 99 female patients compared to 5 males (n¼5) with a mean age of 44±29.8years. Serum Vitamin D levels were found to be low in both RA patients as well as healthy controls. However, it was significantly lower among the patient group compared to healthy controls (p<0.0001). Its immunomodulatory role was assessed from the observation of a significant inverse association between Vitamin D and disease activity scores like DAS 28(r¼0.19,p<0.04) , CDAI(r¼0.25,p¼0.009) and SDAI(r¼0.25,p¼0.009). Further, the levels inversely correlated with inflammatory parameters like ESR and CRP. There was a signifi- cant inverse correlation with HAQ score(r¼0.17,p¼0.06) indicating its value in prognosticating functional as well as disease severity. Conclusion: Low levels of vitamin D contribute significantly to high disease activity and poor functional outcome in patients of RA. P62. Correlation of anti-CCP values with disease activity in patients with rheumatoid arthritis Anish John Padiyara a , Chanchal Gera b , Anna Mani b ; a Department of General Medicine, Dr. SMCSI Medical College, Karakonam, Kerala, India; b Department of General Medicine, Christian Medical College and Hospital, Ludhiana, Punjab, India Introduction: Rheumatoid arthritis(RA) is a common severe chronic inflammatory joint disease. Disease activity can be assessed with DAS28(Disease Activity Score 28) which uses four components: tender joint count, swollen joint count, erythrocyte sedimentation rate(ESR) and Visual analogue scale(VAS) of pa- tient's global/general health. Anti-CCP-antibody, a synthetic cyclic peptide containing citrulline can be detected at an early stage and is a good prognostic tool. In this study, correlation of Anti-CCP values with disease activity(DAS28) in patients with RA was assessed. Methods: This prospective cross sectional observational study was conducted from 01/09/2011 to 30/04/2012. Patients diagnosed to have rheumatoid arthritis for the first time(2010 ACR-EULAR classification criteria)attending Rheumatology clinic were included. Anti-CCP values were determined using ECLIA(Elec- trochemiluminescence Immunoassay) on Elecsys /Cobase immunoassay analyser.DAS28 score was correlated with anti-CCP values using Wilcoxon signed- rank test. Results: Mean DAS28 score is 5±1.3. Mean anti-CCP is 319.01±179.90. p-value calculated was found to be <0.0001 which is significant. Anti-CCP values in patients with low disease activity(DAS28<3.2) was compared. Mean anti-CCP is 52±45.75. p-value is 0.823 showing no significant relationship. Similar test in patients with moderate disease activity(DAS28-3.2 to 5.1) showed significant correlation with p-value of <0.0001. Mean anti-CCP is 236.63±139.94. In patients with high disease activity(DAS28 >5.1), mean anti-CCP is 428.74±151.79. p-value detected is 0.945, showing no significant correlation. Conclusion: There is significant correlation between anti-CCP values and DAS28. Anti-CCP can be used as corroborating marker of disease activity in RA. This significance of increasing titres with DAS28 score was more evident in patients with moderate disease activity. indian journal of rheumatology 9 (2014) S7 eS67 S24