A SNP mutation affects rhizomania-virus content of sugar beets grown on resistance-breaking soils Chiara Broccanello . J. Mitchell McGrath . Lee Panella . Kelley Richardson . Andrew Funk . Claudia Chiodi . Filippo Biscarini . Valeria Barone . Andrea Baglieri . Andrea Squartini . Giuseppe Concheri . Piergiorgio Stevanato Received: 18 June 2017 / Accepted: 12 December 2017 Ó Springer Science+Business Media B.V., part of Springer Nature 2017 Abstract Rhizomania is one of the most devastating biotic stresses affecting sugar beet (Beta vulgaris L.). It is caused by Beet necrotic yellow vein virus (BNYVV) vectored by the plasmodiophorid Polymyxa betae K. The only means available to control the disease is the use of genetically resistant varieties. ‘‘Rizor’’ or ‘‘Holly’’ (Rz1) and WB42 (Rz2) have been the most widely used resistance sources in the commercial varieties. Recently, naturally occurring resistance-breaking (RB) rhizomania strains have been identified causing major concerns. The aim of this study was to identify SNP mutations that show associations with resistance to rhizomania in sugar beet plants grown under resistance-breaking (RB)- BNYVV soils. Rhizomania virus content was evalu- ated by indirect triple-antibody sandwich-ELISA within two F 2 segregating populations respectively grown on an AYPR and IV-BNYVV strain infected soils. Bulked segregant analysis (BSA) was per- formed. The resistant and susceptible plants were genotyped with a 384-SNPs panel. Of the 384 SNPs, SNP249 was found to associate with the resistance both to the AYPR strain (R 2 = 0.37; P = 0.0004) and to the IV-BNYVV (R 2 = 0.09; P = 0.0074). Our results suggested that the SNP249 could be readily applicable for marker-assisted breeding of resistance to AYPR strain of rhizomania. Keywords Sugar beet Á Rhizomania Á Resistance- breaking (RB) strain Á Molecular markers Á SNPs Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10681-017-2098-7) con- tains supplementary material, which is available to authorized users. C. Broccanello Á C. Chiodi Á A. Squartini Á G. Concheri Á P. Stevanato (&) DAFNAE, Universita ` degli Studi di Padova, Legnaro, Italy e-mail: stevanato@unipd.it J. M. McGrath Á A. Funk USDA-ARS Sugarbeet and Bean Research, East Lansing, MI, USA L. Panella USDA-ARS Sugarbeet Research, Fort Collins, CO, USA K. Richardson USDA-ARS Sugarbeet Research, Salinas, CA, USA F. Biscarini CNR-IBBA, Milan, Italy V. Barone Á A. Baglieri Di3A, Universita ` di Catania, Catania, Italy 123 Euphytica (2018) 214:14 https://doi.org/10.1007/s10681-017-2098-7