Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Tue, 09 Oct 2018 01:03:41 Ornithinimicrobium murale sp. nov., isolated from an indoor wall colonized by moulds P. Ka ¨ mpfer, 1 S. P. Glaeser, 1 J. Scha ¨ fer, 1 N. Lodders, 1 K. Martin 2 and P. Schumann 3 Correspondence Peter Ka ¨ mpfer peter.kaempfer@umwelt.uni- giessen.de 1 Institut fu ¨ r Angewandte Mikrobiologie, Justus-Liebig-Universita ¨ t Giessen, Goethestrasse 58, D-35392 Giessen, Germany 2 Leibniz-Institut fu ¨ r Naturstoff-Forschung und Infektionsbiologie e. V., Hans-Kno ¨ ll-Institut-, Beutenbergstr. 11a, D-07745 Jena, Germany 3 Leibniz-Institut DSMZ–Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, D-38124 Braunschweig, Germany A Gram-positive, non-spore-forming actinobacterium (01-Gi-040 T ) isolated from an indoor wall was studied to examine its taxonomic position. The isolate formed a very rudimentary substrate- mycelium that fragmented into rod-shaped to coccoid cells. On the basis of the 16S rRNA gene sequence similarity studies, strain 01-Gi-040 T was shown to belong to the genus Ornithinimicrobium closely related to Ornithinimicrobium kibberense K22-20 T (97.1 %), Ornithinimicrobium humiphilum DSM 12362 T (96.2 %) and Ornithinimicrobium pekingense LW6 T (96.1 %). A close relationship was also found with Arsenicicoccus bolidensis CCUG 47306 T (95.9 %) and Arsenicicoccus piscis Kis4-19 T (95.7 %) and a moderate relationship to the type strains of the genus Serinicoccus (94.0–94.1 %). The predominant menaquinone of strain 01-Gi-040 T was MK-8(H 4 ). The peptidoglycan contained ornithine as the diagnostic diamino acid. The polar lipid profile consisted of the lipids phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, an unknown aminolipid and two unknown phosphoglycolipids. The major fatty acids iso-C 15 : 0 , iso-C 16 : 0 and iso-C 17 : 0 were consistent with the fatty acid patterns reported for members of the genus Ornithinimicrobium. The results of DNA–DNA hybridizations, physiological and biochemical tests allowed phenotypic differentiation of strain 01-Gi-040 T from the three recognized species of the genus Ornithinimicrobium. Strain 01-Gi-040 T represents a novel species of the genus Ornithinimicrobium, for which we propose the name Ornithinimicrobium murale sp. nov., with the type strain 01-Gi-040 T (5DSM 22056 T 5CCM 7610 T ). Several genera of actinomycetes containing ornithine as the diagnostic diamino acid in their cell-wall peptidoglycan are assigned to the families Cellulomonadaceae (Cellulomonas, Rarobacter) and Microbacteriaceae (Microbacterium, Curto- bacterium). In addition, three genera, namely Ornithi- nicoccus, Ornithinimicrobium and Serinicoccus, have been proposed to harbour organisms containing ornithine in the peptidoglycan (Groth et al., 1999, 2001; Yi et al., 2004). The differentiation of these three genera was based on the peptidoglycan structure, polar lipid pattern, fatty acid profile and morphology as well as 16S rRNA gene analysis (Groth et al., 1999, 2001; Yi et al., 2004). Here we report the polyphasic characterization of an isolate containing ornithine in the peptidoglycan and propose a novel species of the genus Ornithinimicrobium. Strain 01-Gi-040 T was isolated from a cellar wall in Germany that was colonized by moulds (Scha ¨fer et al., 2010). The isolation of bacteria from a sample of the material was conducted as described by Ka ¨mpfer et al. (2009). Strain 01-Gi-040 T was maintained on organic medium M79 (www.dsmz.de, Medium 426) and preserved at 280 uC as described previously (Ka ¨mpfer et al., 2009). Cell and colony morphology on M79, nutrient agar (Difco), International Streptomyces Project media 2 and 3 (ISP 2 and ISP 3), Shirling & Gottlieb, 1966) and GYM agar (www.dsmz.de, Medium 65) were determined by phase- contrast microscopy (Axio scope, Carl Zeiss Jena) and by stereomicroscopy (Stemi 2000, Carl Zeiss Jena). Cells of five-day-old liquid cultures of strain 01-Gi-040 T were small irregular rods. After transfer to fresh liquid medium or agar The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 01-Gi-040 T is FR874098. Three supplementary figures are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2013), 63, 119–123 DOI 10.1099/ijs.0.035873-0 035873 G 2013 IUMS Printed in Great Britain 119