Zoonoses Public Health. 2020;00:1–15. wileyonlinelibrary.com/journal/zph | 1 © 2020 Blackwell Verlag GmbH Received: 14 May 2019 | Revised: 14 May 2020 | Accepted: 21 May 2020 DOI: 10.1111/zph.12746 ORIGINAL ARTICLE Qualitative risk assessment of transmission pathways of highly pathogenic avian influenza (HPAI) virus at live poultry markets in Dhaka city, Bangladesh SK Shaheenur Islam 1,2 | Holy Akwar 3 | Md. Mehedi Hossain 1 | Md. Abu Sufian 1 | Md. Zakiul Hasan 3 | Shovon Chakma 3 | Tongkorn Meeyam 4,5 | Warangkhana Chaisowwong 4,5 | Veerasak Punyapornwithaya 5 | Nitish C. Debnath 3 | Eric Brum 3 | Duangporn Pichpol 4,5 1 Department of Livestock Services, Krishi Khamar Sarak, Farmgate, Dhaka, Bangladesh 2 Master of Science in Veterinary Science (International), Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand 3 Emergency Centre for Transboundary Animal Diseases (ECTAD) of Food and Agriculture Organization of United Nations (FAO-UN), Dhaka, Bangladesh 4 Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand 5 Faculty of Veterinary Medicine, Veterinary Public Health Centre for Asia Pacific (VPHCAP), Chiang Mai University, Chiang Mai, Thailand Correspondence Duangporn Pichpol, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand. Email: duangporn.p@cmu.ac.th Funding information This research was funded by FAO-ECTAD Bangladesh and Chiang Mai University, Thailand. Abstract Analysis of environmental samples obtained from the Live Poultry Markets (LPMs) of Dhaka City, Bangladesh, has revealed that the highest degree of prevalence of highly pathogenic avian influenza A (HPAI, H5N1), besides other subtypes of the LPAI virus, poses the plausible risk of transmission of these viruses between human and poultry species. The present study was conducted using the OIE risk analysis framework to assess the risk level of each pathway successively. The estimated risk parameters were integrated towards to obtain the overall risk level for each specific HPAI trans- mission pathway using the matrix adapted by Cristobel Zepeda accompanying other expert consultations. The relevant data obtained from published and unpublished sources, together with survey data of field observations, were used to formulate and confirm the risk pathways and their associated risks. The results revealed that the risk of the release of the HPAI virus was medium when exposure was high. Additionally, the consequence would be considered very high with a medium degree of uncer- tainty for all parameters. Ultimately, the overall risk for transmission was estimated as medium with a medium degree of uncertainty. The findings of this study reveal that there is a significant threat that HPAI virus transmission could occur among poul- try and humans and effectively sustain within the environment of the LPMs. Our findings are primarily focused on public health considerations, the hygienic slaughter of poultry and the relevant cleaning and sanitation practices conducted in the LPMs to support evidence-based decision-making processes. The findings of the study have the potential to be used to formulate effective risk reduction measures and can be further adapted in low-resource settings without major infrastructural changes required of the LPMs. All of which would reduce the risk of HPAI virus release and further lessen the degree of exposure and transmission in established LPMs. KEYWORDS avian influenza virus, Bangladesh, HPAI, live poultry markets, qualitative risk assessment, spillover