181 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ISSN: 2583-4053 Volume-1 Issue-4 || October 2022 || PP. 181-186 https://doi.org/10.55544/jrasb.1.4.26 Journal for Research in Applied Sciences and Biotechnology www.jrasb.com Synergistic Efficiency Between Types of Fungi and Algae for Wastewater Treatment Wassein Jassim Al-Juburi 1 , Mohammad Ibrahim Khalil 2 and Mira Ausamam Al-Katib 3 1 College of Environmental Science and Technology, University of Mosul, IRAQ. 2 College of Environmental Science and Technology, University of Mosul, IRAQ. 3 Department of Biology, College of Environmental Science and Technology, University of Mosul, IRAQ. 1 Corresponding Author: wassein.20evp9@student.uomosul.edu.iq www.jrasb.com || Vol. 1 No. 4 (2022): October Issue Received: 24-09-2022 Revised: 15-10-2022 Accepted: 25-10-2022 ABSTRACT The study aims to demonstrate the efficiency of the synergy between types of fungi and algae for wastewater treatment. Samples are collected from the waters of Wadi al-Kharazi inside the University of Mosul. Four genera of organisms, two sexes of fungi, and two sexes of algae are used for the purpose of reacting. Examinations are conducted for water treated with fungi and algae, as 8 treatments are used compared to the control treatment that contains water alone. The pH function, bicarbonate ions, and sulfates with the two elements iron and copper are measured. The results of the bicarbonate examination show that the best biological treatment is in the mixed culturing of the alga Spirogyra maxima and the fungus Trichoderma asperallum. The average concentration during the incubation periods is 162.8 mg.l - 1 compared to the average control treatment ranging 204.9 mg.l -1 . The results of the sulfate examination show a decrease in concentration for all treatments compared to the control treatment. The best biological treatment in the mixed culturing is between the alga Spirogyra maxima and the fungus Mucor racemosus, as the removal rate reached 48% compared to the average of the control treatment. Examinations have demonstrated the synergy between fungi and algae in increasing the efficiency of pollutant removal rather than using both separately. Keywords- Phosphate, removal, Molecular, Diagnostics, Fungi. I. INTRODUCTION Wastewater pollution is a growing global problem resulting from rapid industrialization, and has been largely unrecognized, with more than 50% of the world's population living in coastal cities (Abaya et al, 2018). Untreated wastewater is introduced to water assets from accidental spills of failed treatment plants. These include sewer overflows, broken sewer pipes, clogged lift stations, and effluent discharged from on-site sewage disposal systems. From an environmental point of view, it is regarded a complex problem as it contains a mixture of high levels of hazardous pollutants. Wastewater contains pathogens (bacteria and viruses), organic and inorganic compounds, endocrine disruptors and hydrocarbons (Ahmed et al, 2018). The applications of using fungi in biological treatment are known as mycoremediation. Fungi are microorganisms with unique properties having the ability to produce various degrading enzymes which has led to their use in biological treatments. Its enzymes have been used to analyze pollutants more in bioremediation applications compared to bacteria due to their ability to overcome some environmental aspects such as the toxicity of persistent organic compounds as well as heavy metals that may limit or prevent biodegradation by bacteria (Sankaran et al., 2010). Moreover, fungi are apical organisms that obtain carbon and energy sources through the decomposition of organic matter by hydrolytic enzymes (Verma et al., 2017). The use of algae in the biological treatment of water is one of the best methods used in the treatment as