The Effect of Soaking Seed with Rizobacteria
Pseudomonas Alcaligenes to the Growth of Swamp
Cabbage Ipomoea Reptans Poir
I Ketut Widnyana*, Maksimus Ngga, and Putu Lasmi Yuliyanthi Sapanca
Laboratory of Agro Technology, University of Mahasaraswati, India
Submission: February 15, 2017; Published: April 25, 2017
*
Corresponding author: I Ketut Widnyana, Laboratory of Agro Technology Faculty of Agriculture, University of Mahasaraswati, India,
Email:
Introduction
Swamp cabbage (Ipomoea reptans Poir) is a vegetable that
has economic value and widespread in Southeast Asia. Swamp
cabbage is generally consumed by the people of Indonesia and
can be one of the restaurant’s menu [1]. Swamp cabbage is a plant
that is relatively resistant to drought and has a broad adaptability
to various environmental conditions plants, easy maintenance and
has a short harvest period [2]. Swamp cabbage commonly grown in
home gardens and some small intensively planted on dry land, so as
to optimize the production of swamp is still lacking. Swamp cabbage
contains complete nutrition, including protein, fat, carbohydrates,
fiber, calcium, phosphorus, iron, sodium, potassium, vitamin
A,B,C, and carotenoids [3]. Additionally, swamp cabbage serves as
medicinal plants to cure constipation, soothe the nerves and the
drug hemorrhoids [4]. Swamp cabbage production in Indonesia can
reach 50.000-60.000 kg ha-1 [5]. Cultivated Swamp cabbage for 0.10
ha spent 16kg of seed planting swamp cabbage however slightly
results are compared with other crops [6]. From the social and
economic aspects, swamp has good prospects if developed towards
agribusiness, but it does require effort in planting efficiency.
The use rizobacteria (Plant Growth Promoting Rhizobacteria
(PGPR)) as a biological fertilizer is the contribution of biotechnology
in crop productivity improvement efforts. This was achieved by
nutrient mobilization, growth hormone production, nitrogen
fixation or activation of the mechanism of disease resistance [7,8].
Therefore, the evaluation of the ability of local Rizobacteria as
bacterial growth driver needs to be done. If proven effective, the
local Rizobacteria can be used as an alternative biological fertilizer
(biofertilizer) on the cultivation of swamp cabbage in Indonesia.
Efforts to reduce the use of synthetic fertilizers and pesticides are
needed in moving towards environmentally sustainable agriculture.
Lately, attention has focused on biological resources in improving
health (resistance) of plants, through the role of beneficial
soil microbes. Microbes that are beneficial to plants, such as
Pseudomonas spp of the rizobacteria group can serve as fertilizer, as
a means of biological control of plant pathogens and improve plant
resistance (induced systemic resistance (ISR) [9].
Rizobacteria is a group of bacteria with the plant root zone
habitat (rizosphere) which has been researched and proven to
Agri Res & Tech: Open Access J 6(1): ARTOAJ.MS.ID.555679 (2017) 0023
Research Article
Volume 6 Issue 1 - April 2017
DOI: 10.19080/ARTOAJ.2017.06.555679
Agri Res & Tech: Open Access J
Copyright © All rights are reserved by I Ketut Widnyanass
Abstract
Research of “The effect of soaking seeds with the bacteria Pseudomonas alcaligenes to the growth of swamp cabbage (Ipomoea reptans Poir)”
was conducted to determine the effect of seed soaking with suspense of P. alcaligenes isolate KtS1, TrN2 and TmA1 to the growth of swamp
cabbage. This research is the development of research that has been done before on tomato plants. This study was designed with a Randomized
Block Design and data analysis using SPSS v.17 for windows. The treatments were three types of isolates of P. alcaligenes, soaking time, and
growing medium. The parameters observed were germination and other growth parameters. The results showed that seed soaking treatment
with suspense P. alcaligenes cause germination 25% faster, higher crop up 24.4%, number of leaves more up to 23.15%, longer stems to 25%,
longer roots up 46.90%, fresh stem weight higher up to 67.07%, dry weight oven stem higher up 84.21% if compared with control. The best
treatment response was TrN2.6NB, soaking seeds of swamp cabbage with P. alcaligenes TrN2 for 60 minutes on medium NB (Natrium broth).
Keywords: Seed soaking; Pseudomonas alcaligenes; Rizobacteria; Swamp cabbage