_______________________________________________________________________________________________________________________________ Open Access Maced J Med Sci. 2018 Nov 25; 6(11):2097-2100. 2097 ID Design Press, Skopje, Republic of Macedonia Open Access Macedonian Journal of Medical Sciences. 2018 Nov 25; 6(11):2097-2100. https://doi.org/10.3889/oamjms.2018.483 eISSN: 1857-9655 Clinical Science Influence of Combined Therapy on Generation of Neutrophil Extracellular Traps in Patients with Cervical Cancer Yuriy Fomenko 1 , Yevgeniya Kolesnikova 2 , Irina Beynikova 2 , Larissa Muravlyova 2* , Valentina Sirota 1 , Ryszhan Bakirova 3 1 Department of Oncology, Karaganda State Medical University, Karaganda, Kazakhstan; 2 Department of the Biological Chemistry, Karaganda State Medical University, Karaganda, Kazakhstan; 3 Department of Internal Diseases, Karaganda State Medical University, Karaganda, Kazakhstan Citation: Fomenko Y, Kolesnikova Y, Beynikova I, Muravlyova L, Sirota V, Bakirova R. Influence of Combined Therapy on Generation of Neutrophil Extracellular Traps in Patients with Cervical Cancer. Open Access Maced J Med Sci. 2018 Nov 25; 6(11):2097-2100. https://doi.org/10.3889/oamjms.2018.483 Keywords: Neutrophil Extracellular Traps; NETs; Cervical Cancer *Correspondence: Larissa Muravlyova. Department of the Biological Chemistry, Karaganda State Medical University, Karaganda, Kazakhstan. E-mail: lem2403@mail.ru Received: 10-Oct-2018; Revised: 06-Nov-2018; Accepted: 07-Nov-2018; Online first: 20-Nov-2018 Copyright: © 2018 Yuriy Fomenko, Yevgeniya Kolesnikova, Irina Beynikova, Larissa Muravlyova, Valentina Sirota, Ryszhan Bakirova. This is an open- access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0) Funding: This research did not receive any financial support Competing Interests: The authors have declared that no competing interests exist Abstract BACKGROUND: According to several authors, neutrophil extracellular traps (NETs) play an important role in the mechanisms of cancer development and metastatic processes, which allows them to be considered as a potential new target for the treatment of cancer. AIM: To investigate the presence of extracellular neutrophil traps in the blood of patients with cervical cancer on the background of the combined treatment. MATERIALS AND METHODS: The study was conducted in 28 patients with cervical cancer. Group 1 received only radiation therapy; Groups 2-radiation therapy with ftorafur; Group 3-radiation therapy with cisplatin. To determine the number of spontaneous extracellular neutrophilic traps in the blood of the examined individuals, we used a technique of I.I. Dolgushin and Yu.S. Andreeva. RESULTS: Peripheral blood neutrophils in 53.57% (33.87; 72.49) of cervical cancer patients showed the ability to generate NETs before treatment. The ability to form NETs was observed in neutrophils isolated from 66.67% (9.43; 99.16) patients of the Group 1. After radiation therapy with ftorafur, the ability of blood neutrophils to form NETs was observed in 50% (1.26; 98.74) of cervical cancer patients. After radiotherapy with cisplatin, 37.50% (15.20; 64.57) of patients were found to have NETs formation CONCLUSION: The ability to form NETs varied greatly after radiotherapy. The addition of chemotherapy drugs to radiation therapy did not increase the percentage of NETs in the blood of patients with cervical cancer but stimulated the appearance of basophil extracellular traps. Introduction The issue of the role of neutrophil extracellular traps (NETs) in oncology is at the stage of the study. An in vitro study of the formation of NETs after stimulation of neutrophils obtained from patients with colorectal cancer and healthy donors of the same age group was carried out. It was found that neutrophils of patients' blood formed spontaneous NETs. A significant increase in the number of NETs in response to the stimulus was recorded in patients with postoperative complications. There was no relationship between the number of NETs, the stage and the localisation of a tumour. According to the authors of the study, these results allow us to consider the formation of NETs as a potential target for therapy [1]. At the same time, according to other researchers, neutrophils isolated from the blood of patients with uterine cancer (stage Ia) had a reduced ability to form traps in response to stimulation by yeast cells [2]. It was suggested that the formation of NETs could be one of the mechanisms of tumour development. The basis for this assumption was the evidence that the granulocyte colony-stimulating factor, which is produced by many tumours and is determined in the blood of cancer patients, triggers the formation of NETs [3].