S176 Indian Journal of Pharmaceutical Education and Research | Vol 55 | Issue 1 [Suppl] | Jan-Mar, 2021 Original Artcle www.ijper.org Evaluation of Some Phenolic Acids in Diabetic Neuropathy Shubhangi Pawar 1, *, Aman Upaganlawar 2 , Chandrashekhar Upasani 2 1 Department of Pharmacology, MGV’s Pharmacy College, Panchavati, Nashik, Maharashtra, INDIA. 2 Department of Pharmacology, SNJB’s SSDJ College of Pharmacy, Chandwad, Nashik, Maharashtra, INDIA. ABSTRACT Background: Streptozotocin (STZ) induced neuropathy is widely used preclinical model for diabetic neuropathy (DN). DN is majorly resulted due to nitrosative and oxidative stress induced by hyperglycemia. Phenolic acids are polyphenols with free radical scavenging anti-inflammatory and neuroprotectiveaction. Methods: In this study STZ (55mg/kg, i.p) was administered in male Wistar rats and animals with hyperglycemia (fasting blood glucose ≥ 200mg/dl) were used for further study. Behavioural changes cold allodynia, mechanical hyperalgesia, heat hyperalgesia, mechanical allodynia were assessed weekly. Motor Nerve Conduction Velocity (MNCV) was also evaluated. Reduced Glutathione and Malondialdehyde were estimated to indicate oxidative stress. C-Reactive Protein (CRP), Insulin assay, serum electrolytes (Na + , K + ), TNF-α, IL-6 and INF-γ were also estimated. Isolated sciatic nerve was histopathologically studied to support the results. Results: Treatment with syringic acid (SY) 12.5, 25, 50 mg/kg and of Sinapic acid (SP) 5, 10, 20 mg/kg orally for 5 weeks has shown to reduce blood glucose level. Behavioural changes were found to be improved weekly by SY and SP in dose dependent manner. 5 weeks treatment with SY and SP was able to increase antioxidant GSH and reduce MDA level in cell. Gabapentin, SY and SP treated animals have shown decrease in TNF-α, IL-6 and INF-γ and CRP. Insulin and serum electrolytes were found to be normalised in treated groups. Histopathological study has revealed protective effect of gabapentin, SY and SP by showing reverted neuronal damage. Conclusion: In conclusion, syringic acid and sinapic acid have antihyperglycemic, antioxidant and neuroprotective effect in diabetic neuropathy. Key words: Phenolic acids, Neuropathy, Hyperalgesia, Allodynia, Nerve conduction velocity, Antioxidants, Cytokines. DOI: 10.5530/ijper.55.1s.48 Correspondence: Mrs. Shubhangi H Pawar Department of Pharmacology, MGV’s Pharmacy College, Panchavati, Nashik, Maharashtra, INDIA. Phone no: +91 9823192936 Email id: shpawar2009@ gmail.com Submission Date: 30-06-2020; Revision Date: 24-12-2020; Accepted Date: 03-02-2021 INTRODUCTION Streptozotocin (STZ) is well established and reliable to induce diabetic neuropathy. STZ is nitrosoureas antibiotic used as anticancer. STZ selectively destructs pancreatic ß cells at dose of 45 to 70 mg/kg (i.v or i.p) and after 3-4 days, in rats causes hyperglycemia to induce diabetes. 1 Diabetic neuropathy is majorly resulted due to nitrosative and oxidative stress induced by hyperglycemia. Thus formed reactive oxygen species (ROS) can cause sensory and motor nerve conduction defects. 2 In animal models of diabetes mellitus (DM), STZ is suitably used to study disease pathogenesis and its complications. 3 As modern medicines prominently show adverse effects, natural drugs are safer therapeutic alternative to treat neuropathy. Various plants and their phytoconstituents are selectively studied in the treatment of neuropathy in rats. 4 Phenolic acids are polyphenols, having anti-infammatory and free radical scavenging action, have been proven as neuroprotective. 5 In accordance with these effects of various phenolic acids, unravelled members of this class can be evaluated through rational research plan. Syringic acid (SY) is useful in treatment of diabetes, cardiovascular diseases, cancer and cerebral ischemia. It is having antioxidant,