How to Cite: Caf, F., Algül, S., and Köprücü, S., (2022). Determination of The Presence of Nucleobindin-2/Nesfatin in Cyprinus Carpio (Linnaeus, 1758) and Cyprinion Macrostomus (Heckel, 1843). Ecological Life Sciences, 17(4):179-186, DOI: 10.12739/NWSA.2022.17.4.5A0177. Ecological Life Sciences Status : Research Article ISSN: 1308 7258 Received: 16.07.2022 Article ID: 5A0177 Accepted: 25.10.2022 Fatma Caf Bingöl University, f.baydas23@hotmail.com, Bingöl-Türkiye Şermin Algül Van Yüzüncü Yıl University, serminalgul@hotmail.com, Van-Türkiye Sibel Köprücü Fırat University, skoprucu@firat.edu.tr, Elazığ-Türkiye DOI http://dx.doi.org/10.12739/NWSA.2022.17.4.5A0177 ORCID ID 0000-0002-6565-3550 0000-0002-0363-4848 0000-0003-2489-3619 Corresponding Author Sibel Köprücü DETERMINATION OF THE PRESENCE OF NUCLEOBINDIN-2/NESFATIN IN Cyprinus carpio (Linnaeus, 1758) AND Cyprinion macrostomus (Heckel,1843) ABSTRACT Nucleobindin-2 protein was also termed as nesfatin (for NEFA/NUCB2-encoded satiety and fat-influencing proteins). The aim of this study is to determine to find out whether NUCB2/nesfatin in the blood serum of Cyprinus carpio and Cyprinion macrostomus by enzyme- linked immuno assay (ELISA). Nesfatin level was analysed using Fish Nesfatin ELISA kit (Cat. No: MBS013992). Results were compared between two species and between sexes of each species. In addition, nesfatin levels were also compared with the body weight and length of both C. carpio and C. macrostomus. There has been no study the Nucleobindin- 2/Nesfatin described in C. macrostomus, C. carpio in the literature. Keywords: NUCB2/Nesfatin, Cyprinus carpio, Cyprinion macrostomus, Blood Serum, ELISA 1. INTRODUCTION Nucleobindin-2/Nesfatin is a 50-kDa protein hormone. It is consist of 396 amino acids, with very high amino acid sequence homology among rat, mouse, and human species [1]. It is suggested that nucleobindin-2 is divided by prohormone convertases into various active peptides, nesfatin-1 (1-82 aminoacid), nesfatin-2 (85–163 amino acid), and nesfatin-3 (166–396 amino acid) [2 and 3]. Of these three peptides, only nesfatin-1 has been shown to have biological functions. The main functions of nesfatin-1 is involved in the regulation of miscellaneous physiological metabolism, including feeding by reducing feed intake [3, 4, 5, 6, 7, and 8], locomotion, reproduction, stress modulation termogenesis [9, 10, and 11] and energy homeostasis [4, 5, 12, 13, 14, 15, 16, and 17]. In a number of animals and humans are present NUCB2 and the nesfatin-1 region displays a high identity among reported NUCB2 sequences [18]. The predicted nesfatin-1 and 2 in Carassius auratus are highly conserved and 82 and 81 amino acids long respectively. Nesfatin-3 is longer in Carassius auratus (309 amino acids) than in rat (231 amino acids) [6]. In fish, two isoforms of NUCB2 (NUCB2A and NUCB2B) exist and nesfatin-1 has been identified Schizothorax prenanti [19], Danino rerio [8 and 20], Carassius auratus [2, 6, and 8], female Oncorhynchus mykiss [21] and Alburnus tarichi [22] most abundantly found in liver but least found in gut brain and tissues. The Both NUCB2A and NUCB2 mRNAs in the Danino rerio were most abundant in the liver but less expression was found in other tissues including the gut and brain. Additionally NUCB2 mRNA expression and protein content has been demonstrated several tissues including the