523 P-ISSN: 2304-3075; E-ISSN: 2305-4360 International Journal of Veterinary Science www.ijvets.com; editor@ijvets.com Research Article DOI: 10.37422/IJVS/20.074 Treasing Study on Tylvamyco ® as a Novel Immunomodulatory Medication for Broiler Chickens Mustafa Bastamy 1 , Ismail Raheel 2 , Hany Ellakany 3 and Ahmed Orabi 4* 1 Department of Poultry and Rabbits Disease, Faculty of Veterinary Medicine, Cairo University, Egypt 2 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suief University, Egypt 3 Department of Poultry and Rabbits Disease, Faculty of Veterinary Medicine, Damanhour University, Egypt 4 Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt *Corresponding author: drorabi2012@yahoo.com; orabi.vet@cu.edu.eg Article History: 20-083 Received: March 30, 2020 Revised: June 24, 2020 Accepted: July 04, 2020 Abstract Present study aims at the evaluation of the efficacy of Tylvamyco ® as a new macrolides generation in control of avian mycoplasmosis in broilers chickens with special attention to its immunomodulatory effects. A total of 500 a-day-old broiler Ross 308 chicks were equally subdivided into two treatments of 250 birds in each. The Tylvamyco ® treated group, and the control non treated group were kept in a separate house. Blood samples and tracheal tissues collected at one day old and also each week till the end of the trials for isolation M. gallisepticum and also measuring the immune status of the experimental chicks. M. gallisepticum occurrence rate in broilers chickens was 12% which confirmed by PCR. The minimal inhibitory concentration values Tylvamyco ® against recovered 12 M. gallisepticum isolates standard strain showed that the Tylvamyco ® has MIC90 value of 0.008. In the Tylvamyco ® treated group the immune status profiles record that there are marked increase in the immunological parameters by age as; HI test results for Mycoplasma, NDV, AI, INF-γ conc., IL-6 conc., phagocytic cell count, nitric oxide conc. and lysozyme conc. at 1, 15 and 30 day old, respectively. The molecular analysis of CXCL8 gene as an indicator for inflammation reduction potency in In the Tylvamyco ® treated group by using real-time PCR showed that the cycle threshold of CXCL8 gene reduced by age from 13.6 to 10.7 at 15 and 30 day old with fold change 0.57 and 1.4, respectively. Performance parameter in Tylvamyco ® treated group was 3.22kg/bird with mean weight gain 2.33kg/bird and FCR 1.4. The mortality rate was 5%. We concluded that Tylvamyco ® acts as a potent immunomodulatory medicine in broilers. Key words: Tylvamyco, Mycoplasmas, Immunomodulation, Broiler chickens. INTRODUCTION Avian mycoplasmosis causes considerable economical losses to the poultry industry, especially in chickens all over the world. In broilers, M. gallisepticum cause a reduction in weight gain, a decrease in feed conversion efficiency, an increased mortality rate, and increased condemnations at slaughter (Ley, 2008). Even with the monitoring and control programs in place, many chicken flocks become infected vertically and horizontally (Bradbury, 2001). The three main approaches for the control of the disease are eradication followed by prevention, vaccination or medication. While eradication and vaccination provide long-term solution for the control of mycoplasmosis, medication can be a prompt and effective tool to reduce the economic losses by mitigating egg transmission and clinical signs (Kleven, 2008). However, antibiotic susceptibility profile should first be determined to maximize treatment efficacy (Landman et al., 2008). Immunity is split into innate and adaptive systems. Although it was once thought that the inflammation was not very highly regulated, further research into the key players and processes that make up the phenomenon have revealed that there is complex web of processes that coordinate this innate immune response. As the first line of the innate immune response, including inflammation is necessary to remove an invading pathogen post-infection (Jain and Pasare, 2017). Macrophages are tissue resident phagocytes; similar to neutrophils, they are derived from myeloid precursors and play a key role in antimicrobial activity. They also have a more diverse repertoire of functions including tissue surveillance, remodelling and antigen presentation, which helps link innate and adaptive immune responses Cite This Article as: Bastamy M, Raheel I, Ellakany H and Orabi A, 2020. Treasing study on Tylvamyco ® as a novel immunomodulatory medication for broiler chickens. Int J Vet Sci, 9(4): 523-527. www.ijvets.com (©2020 IJVS. All rights reserved)