Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 407-415 407 Original Research Article https://doi.org/10.20546/ijcmas.2020.904.049 Invitro Evaluation of Botanicals and ITKs against Septoria lycopersici Causing Septoria Leaf Spot of Tomato D. R. Sindushree*, Gurudatt M. Hegde and Yashoda R. Hegde Department of Plant Pathology, College of Agriculture, Dharwad University of Agricultural Sciences, Dharwad-580 005, Karnataka, India *Corresponding author ABSTRACT Introduction Tomato (Solanum lycopersicum L.) is one of the most remunerative and widely grown vegetables in the world. It is cultivating extensively for its edible fruits, which are rich source of vitamin A and C. Fruit is bright red due to the pigment lycopene, which is the major carotenoid in tomatoes, exhibits the highest antioxidant activity and it is a beneficial colouring agent for food because of its solid color and nontoxicity (Schulzova and Hajslova, 2007). In India, the crop is being cultivated in a variety of climate, across states of Maharashtra, Andhra Pradesh, Karnataka, West Bengal, Odisha, Bihar, Madhya Pradesh, Uttar Pradesh, Gujarat and Chhattisgarh. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 4 (2020) Journal homepage: http://www.ijcmas.com Tomato (Solanum lycopersicum L.) is one of the commercially important major vegetable crops grown throughout the tropics and subtropics. Septoria leaf spot caused by Septoria lycopersici Speg. is one of the most destructive diseases of tomato and is particularly severe in areas where wet, humid weather persists for extended periods. The bio assay of botanicals and Indigenous technical knowledge (ITKs) forms the prerequisite for the field evaluation. In the present investigation eight botanicals and eight ITKs were evaluated at 5, 10 and 15 per cent concentration against S. lycopersici under laboratory condition. Among the eight botanicals, Allium sativum (Garlic) bulb extract found to be significantly superior over other treatments in inhibiting the mycelial growth (65.10 %) of S. lycopersici followed by Duranta goldiana (Duranta) leaf extract (53.73 %) and lowest inhibition was observed in Tagates erecta (Marigold) leaf extract (23.53 %) at 15 per cent concentration. In vitro spore germination bioassay of ITKs revealed that, highest inhibition (76.67 %) of conidial germination over control was shown by Cow urine which was on par with Panchagavya (73.33 %) and the least inhibition was observed in biodigestor (33.33 %) at 15 per cent concentration. Keywords Tomato, Septoria lycopersici, In vitro, Botanicals, ITKs Accepted: 04 March 2020 Available Online: 10 April 2020 Article Info